Stimulation of the CD2 receptor pathway induces apoptosis in human T lymphotropic virus type I-infected cell lines

Deborah J. Guyot, O. Joseph Trask, Janice M. Andrews, Garret C. Newbound, Michael Dale Lairmore

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We demonstrate that CD2 receptor engagement, but not CD3 cross-linking, induces apoptosis in lymphocytes transformed by human T-cell lymphotropic virus type I (HTLV-I). Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [3H]thymidine incorporation from 25 to 62% in CD2+ HTLV-I- infected lymphocytes. This inhibition was associated with a 20-40% reduction in cell number and viability over a 3-day period, morphologic evidence of apoptosis, and irreversible DNA fragmentation. While cyclosporin A abrogated CD2-mediated proliferation in peripheral blood mononuclear cells, it had no effect on CD2-induced apoptosis in the HTLV-I-infected cell lines. Since HTLV-I is mitogenic to resting lymphocytes through CD2 activation pathways, these results suggest that HTLV-I-infected lymphocytes are primed for apoptosis following additional CD2 stimulation. This CD2-mediated apoptosis might be a factor in immune regulation of HTLV-I-associated diseases or might offer a novel adjunctive approach to treatment.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume11
Issue number4
StatePublished - 1996
Externally publishedYes

Keywords

  • Apoptosis
  • CD2
  • CD3
  • Human T lymphotropic virus type I

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

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