Stimulation of proteoglycan synthesis in explants of porcine articular cartilage by recombinant osteogenic protein-1 (bone morphogenetic protein-7)

Steven A. Lietman, Masaki Yanagishita, T. K. Sampath, A Hari Reddi

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Osteogenic protein-1 (also known as bone morphogenetic protein-7) is a member of the bone morphogenetic protein family. Bone morphogenetic proteins and related members of the TGF-β (transforming growth factor-β) superfamily are involved in the development and repair of bone. Recombinant bone morphogenetic proteins induce the formation of new cartilage and bone at heterotopic sites. We investigated the influence of recombinant osteogenic protein-1 (at doses of three, ten, thirty, or 100 nanograms per milliliter) on the synthesis and release of proteoglycans and the maintenance of a steady-state concentration of proteoglycans in explants of porcine articular cartilage that were maintained in chemically defined serum-free medium. We found a dose-dependent stimulation of proteoglycan synthesis and a concurrent decrease in the rate of release of proteoglycans from the explants. The size of the proteoglycan monomers and the composition of the glycosaminoglycan chains in the untreated articular cartilage were similar to those in the articular cartilage treated with osteogenic protein-1. The capacity of the newly synthesized proteoglycan monomers to form aggregates with exogenous hyaluronic acid was found to be similar to that of proteoglycans in bovine nasal cartilage. Our results demonstrated that osteogenic protein-1 stimulated the synthesis of proteoglycans and diminished the release of proteoglycans from explants of porcine articular cartilage. CLINICAL RELEVANCE: The maintenance and repair of articular cartilage is a formidable challenge in clinical orthopaedics. The stimulation of proteoglycan synthesis by osteogenic protein-1. (bone morphogenetic protein-7) in explants of cartilage maintained in chemically defined serum-free medium implies that recombinant osteogenic protein-1 may play a role in the maintenance of a steady-state concentration of proteoglycans in articular cartilage, a desirable prerequisite for optimum repair of cartilage. Osteogenic protein-1 can initiate the formation of cartilage from mesenchymal cells. Once new cartilage has formed at the site of repair, osteogenic protein-1 also may maintain the synthesis of proteoglycans.

Original languageEnglish (US)
Pages (from-to)1132-1137
Number of pages6
JournalJournal of Bone and Joint Surgery - Series A
Volume79
Issue number8
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 7
Articular Cartilage
Proteoglycans
Recombinant Proteins
Swine
Cartilage
Bone Morphogenetic Proteins
Serum-Free Culture Media
Maintenance
Nasal Cartilages
Bone Development
Transforming Growth Factors
Hyaluronic Acid
Glycosaminoglycans
Orthopedics

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Stimulation of proteoglycan synthesis in explants of porcine articular cartilage by recombinant osteogenic protein-1 (bone morphogenetic protein-7). / Lietman, Steven A.; Yanagishita, Masaki; Sampath, T. K.; Reddi, A Hari.

In: Journal of Bone and Joint Surgery - Series A, Vol. 79, No. 8, 08.1997, p. 1132-1137.

Research output: Contribution to journalArticle

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