Stimulation of osteoclast formation by inflammatory synovial fluid

Iannis Adamopoulos, Lynett Danks, Ichiro Itonaga, Rachel M. Locklin, Afsie Sabokbar, David J P Ferguson, Nicholas A. Athanasou

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Peri-articular bone resorption is a feature of arthritis due to crystal deposition and rheumatoid disease. Under these conditions, the synovial fluid contains numerous inflammatory cells that produce cytokines and growth factors which promote osteoclast formation. The aim of this study was to determine whether inflammatory synovial fluid stimulates the formation of osteoclasts. Synovial fluid from rheumatoid arthritis (RA), pyrophosphate arthropathy (PPA) and osteoarthritis (OA) patients was added to cultures (n=8) of human peripheral blood mononuclear cells (PBMCs) in the presence and absence of macrophage colony-stimulating factor (M-CSF) and the receptor activator of NF-κB ligand (RANKL). Osteoclast formation was assessed by the formation of cells positive for tartrate-resistant acid phosphatase (TRAP) and vitronectin receptor (VNR) and the extent of lacunar resorption. The addition of 10% OA, RA and PPA synovial fluid to PBMC cultures resulted in the formation of numerous multinucleated or mononuclear TRAP+ and VNR+ cells which were capable of lacunar resorption. In contrast to PBMC cultures incubated with OA synovial fluid, there was marked stimulation of osteoclast formation and resorption in cultures containing inflammatory RA and PPA synovial fluid which contained high levels of tumour necrosis factor alpha, a factor which is known to stimulate RANKL-induced osteoclast formation.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalVirchows Archiv
Volume449
Issue number1
DOIs
StatePublished - Jul 2006
Externally publishedYes

Fingerprint

Synovial Fluid
Osteoclasts
Joint Diseases
Integrin alphaVbeta3
Osteoarthritis
Blood Cells
Rheumatoid Arthritis
Cell Culture Techniques
Macrophage Colony-Stimulating Factor Receptors
Bone Resorption
Arthritis
Intercellular Signaling Peptides and Proteins
Tumor Necrosis Factor-alpha
Joints
Cytokines
Ligands
diphosphoric acid

Keywords

  • Crystal
  • Osteoclast
  • Pyrophosphate
  • Rheumatoid arthritis
  • Synovial fluid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Adamopoulos, I., Danks, L., Itonaga, I., Locklin, R. M., Sabokbar, A., Ferguson, D. J. P., & Athanasou, N. A. (2006). Stimulation of osteoclast formation by inflammatory synovial fluid. Virchows Archiv, 449(1), 69-77. https://doi.org/10.1007/s00428-006-0200-y

Stimulation of osteoclast formation by inflammatory synovial fluid. / Adamopoulos, Iannis; Danks, Lynett; Itonaga, Ichiro; Locklin, Rachel M.; Sabokbar, Afsie; Ferguson, David J P; Athanasou, Nicholas A.

In: Virchows Archiv, Vol. 449, No. 1, 07.2006, p. 69-77.

Research output: Contribution to journalArticle

Adamopoulos, I, Danks, L, Itonaga, I, Locklin, RM, Sabokbar, A, Ferguson, DJP & Athanasou, NA 2006, 'Stimulation of osteoclast formation by inflammatory synovial fluid', Virchows Archiv, vol. 449, no. 1, pp. 69-77. https://doi.org/10.1007/s00428-006-0200-y
Adamopoulos I, Danks L, Itonaga I, Locklin RM, Sabokbar A, Ferguson DJP et al. Stimulation of osteoclast formation by inflammatory synovial fluid. Virchows Archiv. 2006 Jul;449(1):69-77. https://doi.org/10.1007/s00428-006-0200-y
Adamopoulos, Iannis ; Danks, Lynett ; Itonaga, Ichiro ; Locklin, Rachel M. ; Sabokbar, Afsie ; Ferguson, David J P ; Athanasou, Nicholas A. / Stimulation of osteoclast formation by inflammatory synovial fluid. In: Virchows Archiv. 2006 ; Vol. 449, No. 1. pp. 69-77.
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