Stimulation of chondrogenesis in limb bud mesoderm cells by recombinant human bone morphogenetic protein 2B (BMP-2B) and modulation by transforming growth factor β1 and β2

Ping Chen, Jill L. Carrington, R. G. Hammonds, A Hari Reddi

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Bone morphogenetic protein 2B (BMP-2B) also called BMP-4 is one of a family of cartilage and bone-inductive proteins derived from bone matrix and belongs to the transforming growth factor β (TGF-β) superfamily. These bone-inductive proteins isolated from adult bone may be involved in bone repair. However, they may also play a role in cartilage and bone formation during embryonic development. To test whether BMP-2B influences cartilage formation by embryonic cells, recombinant human BMP-2B was applied to cultured limb bud mesoderm plated at three different densities. BMP-2B stimulated cartilage formation as assessed by Alcian blue staining and incorporation of radioactive sulfate into sulfated proteoglycans. Cells cultured at all three densities in the presence of 10 ng/ml BMP-2B formed a nearly continuous sheet of cartilage with abundant extracellular matrix and type II collagen. In addition, when cells were cultured in 0.5% serum in the presence of 10 ng/ml of BMP-2B for 5 days there was an increase in alkaline phosphatase as detected by histochemical and biochemical methods. Transforming growth factor β isoforms (TGF-β1 and TGF-β2) inhibited sulfate incorporation into proteoglycans in a dose-dependent manner. This inhibition by TGFβ was overcome by recombinant BMP-2B. This study demonstrates that recombinant BMP-2B stimulates cartilage formation by chick limb bud mesoderm in vitro and is further modulated by TGF-β isoforms.

Original languageEnglish (US)
Pages (from-to)509-515
Number of pages7
JournalExperimental Cell Research
Volume195
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 4
Limb Buds
Chondrogenesis
Transforming Growth Factors
Mesoderm
Cartilage
Bone and Bones
Proteoglycans
Recombinant Proteins
Sulfates
Bone Morphogenetic Protein 5
Cultured Cells
Protein Isoforms
Alcian Blue
Bone Matrix
Collagen Type II
Osteogenesis
Embryonic Development
Extracellular Matrix
Alkaline Phosphatase

ASJC Scopus subject areas

  • Cell Biology

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Stimulation of chondrogenesis in limb bud mesoderm cells by recombinant human bone morphogenetic protein 2B (BMP-2B) and modulation by transforming growth factor β1 and β2 . / Chen, Ping; Carrington, Jill L.; Hammonds, R. G.; Reddi, A Hari.

In: Experimental Cell Research, Vol. 195, No. 2, 1991, p. 509-515.

Research output: Contribution to journalArticle

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