Sterol synthesis by myelinating cultures of mouse spinal cord

David E Pleasure, Seung U. Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We studied the control of sterol synthesis during myelination of central nervous system in culture. Explants of fetal mouse spinal cord were cultured for 3-30 days in vitro (DIV). Myelination was visible by electron microscopy at 3 DIV, and by bright-field light microscopy beginning at 6 DIV. All explants were heavily myelinated by 15 DIV. Specific activity of 2′,3′-cyclic nucleotide-3′-phosphohydrolase (CNP) increased rapidly until 15 DIV, then more slowly. Explant sterol content rose more than threefold between 5 and 30 DIV. The rate of incorporation of [1-14C]acetate into sterol was greatest at 12 DIV, more than double that at 4 and 22 DIV. Specific activity of the mevalonate synthesizing enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) was greatest at 11 DIV, threefold greater than at 6 and 30 DIV. Previous studies in fibroblasts and other non-neural tissues had shown that deprivation of exogenous lipid caused a rapid increase in HMG CoA reductase activity. In contrast, when the spinal cord explants were incubated for 24 h in a lipid-deficient medium, there was either a marked decrease in specific activity of HMG CoA reductase (at 6, 15 and 30 DIV), or no change in enzyme activity (at 11 DIV).

Original languageEnglish (US)
Pages (from-to)117-126
Number of pages10
JournalBrain Research
Volume103
Issue number1
DOIs
StatePublished - Feb 13 1976
Externally publishedYes

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Sterols
Spinal Cord
Oxidoreductases
nucleotidase
Coenzyme A
In Vitro Techniques
Lipids
Mevalonic Acid
Cyclic Nucleotides
Enzymes
Microscopy
Electron Microscopy
Acetates
Central Nervous System
Fibroblasts
Light

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Sterol synthesis by myelinating cultures of mouse spinal cord. / Pleasure, David E; Kim, Seung U.

In: Brain Research, Vol. 103, No. 1, 13.02.1976, p. 117-126.

Research output: Contribution to journalArticle

Pleasure, David E ; Kim, Seung U. / Sterol synthesis by myelinating cultures of mouse spinal cord. In: Brain Research. 1976 ; Vol. 103, No. 1. pp. 117-126.
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