Abstract
We studied the control of sterol synthesis during myelination of central nervous system in culture. Explants of fetal mouse spinal cord were cultured for 3-30 days in vitro (DIV). Myelination was visible by electron microscopy at 3 DIV, and by bright-field light microscopy beginning at 6 DIV. All explants were heavily myelinated by 15 DIV. Specific activity of 2′,3′-cyclic nucleotide-3′-phosphohydrolase (CNP) increased rapidly until 15 DIV, then more slowly. Explant sterol content rose more than threefold between 5 and 30 DIV. The rate of incorporation of [1-14C]acetate into sterol was greatest at 12 DIV, more than double that at 4 and 22 DIV. Specific activity of the mevalonate synthesizing enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) was greatest at 11 DIV, threefold greater than at 6 and 30 DIV. Previous studies in fibroblasts and other non-neural tissues had shown that deprivation of exogenous lipid caused a rapid increase in HMG CoA reductase activity. In contrast, when the spinal cord explants were incubated for 24 h in a lipid-deficient medium, there was either a marked decrease in specific activity of HMG CoA reductase (at 6, 15 and 30 DIV), or no change in enzyme activity (at 11 DIV).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 117-126 |
| Number of pages | 10 |
| Journal | Brain Research |
| Volume | 103 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 13 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Sterol synthesis by myelinating cultures of mouse spinal cord. / Pleasure, David E; Kim, Seung U.
In: Brain Research, Vol. 103, No. 1, 13.02.1976, p. 117-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sterol synthesis by myelinating cultures of mouse spinal cord
AU - Pleasure, David E
AU - Kim, Seung U.
PY - 1976/2/13
Y1 - 1976/2/13
N2 - We studied the control of sterol synthesis during myelination of central nervous system in culture. Explants of fetal mouse spinal cord were cultured for 3-30 days in vitro (DIV). Myelination was visible by electron microscopy at 3 DIV, and by bright-field light microscopy beginning at 6 DIV. All explants were heavily myelinated by 15 DIV. Specific activity of 2′,3′-cyclic nucleotide-3′-phosphohydrolase (CNP) increased rapidly until 15 DIV, then more slowly. Explant sterol content rose more than threefold between 5 and 30 DIV. The rate of incorporation of [1-14C]acetate into sterol was greatest at 12 DIV, more than double that at 4 and 22 DIV. Specific activity of the mevalonate synthesizing enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) was greatest at 11 DIV, threefold greater than at 6 and 30 DIV. Previous studies in fibroblasts and other non-neural tissues had shown that deprivation of exogenous lipid caused a rapid increase in HMG CoA reductase activity. In contrast, when the spinal cord explants were incubated for 24 h in a lipid-deficient medium, there was either a marked decrease in specific activity of HMG CoA reductase (at 6, 15 and 30 DIV), or no change in enzyme activity (at 11 DIV).
AB - We studied the control of sterol synthesis during myelination of central nervous system in culture. Explants of fetal mouse spinal cord were cultured for 3-30 days in vitro (DIV). Myelination was visible by electron microscopy at 3 DIV, and by bright-field light microscopy beginning at 6 DIV. All explants were heavily myelinated by 15 DIV. Specific activity of 2′,3′-cyclic nucleotide-3′-phosphohydrolase (CNP) increased rapidly until 15 DIV, then more slowly. Explant sterol content rose more than threefold between 5 and 30 DIV. The rate of incorporation of [1-14C]acetate into sterol was greatest at 12 DIV, more than double that at 4 and 22 DIV. Specific activity of the mevalonate synthesizing enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) was greatest at 11 DIV, threefold greater than at 6 and 30 DIV. Previous studies in fibroblasts and other non-neural tissues had shown that deprivation of exogenous lipid caused a rapid increase in HMG CoA reductase activity. In contrast, when the spinal cord explants were incubated for 24 h in a lipid-deficient medium, there was either a marked decrease in specific activity of HMG CoA reductase (at 6, 15 and 30 DIV), or no change in enzyme activity (at 11 DIV).
UR - http://www.scopus.com/inward/record.url?scp=0017257854&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017257854&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(76)90691-0
DO - 10.1016/0006-8993(76)90691-0
M3 - Article
C2 - 175882
AN - SCOPUS:0017257854
VL - 103
SP - 117
EP - 126
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -