Stereotyped axon pruning via plexin signaling is associated with synaptic complex elimination in the hippocampus

Xiao Bo Liu, Lawrence K. Low, Edward G. Jones, Hwai-Jong Cheng

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Plexin signaling is required for stereotyped pruning of long axon collaterals in the vertebrate CNS; however, a cellular basis for plexins on stereotyped pruning has not been determined. Using quantitative electron microscopy and immunocytochemistry, we found that infrapyramidal mossy fiber axon collaterals form transient synaptic complexes with basal dendrites of CA3 pyramidal cells in the early postnatal mouse hippocampus. At later postnatal ages, these synaptic complexes stop maturing and are removed before stereotyped pruning by a mechanism that does not involve axon degeneration and glial cell engulfment. In knock-out mice that lack plexin-A3 signaling, the synaptic complexes continue to mature, and, as a result, the collaterals are not pruned. Thus, our results suggest that intact plexin-A3 signaling contributes to synaptic complex elimination, which is associated with stereotyped axon pruning.

Original languageEnglish (US)
Pages (from-to)9124-9134
Number of pages11
JournalJournal of Neuroscience
Volume25
Issue number40
DOIs
StatePublished - Oct 5 2005

Fingerprint

Neuronal Plasticity
Hippocampus
Axons
Pyramidal Cells
Dendrites
Knockout Mice
Neuroglia
Vertebrates
Electron Microscopy
Immunohistochemistry
plexin

Keywords

  • Axon pruning
  • Granule cell
  • Hippocampus
  • Mossy fiber
  • Plexin
  • Semaphorin
  • Synaptic complex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Stereotyped axon pruning via plexin signaling is associated with synaptic complex elimination in the hippocampus. / Liu, Xiao Bo; Low, Lawrence K.; Jones, Edward G.; Cheng, Hwai-Jong.

In: Journal of Neuroscience, Vol. 25, No. 40, 05.10.2005, p. 9124-9134.

Research output: Contribution to journalArticle

Liu, Xiao Bo ; Low, Lawrence K. ; Jones, Edward G. ; Cheng, Hwai-Jong. / Stereotyped axon pruning via plexin signaling is associated with synaptic complex elimination in the hippocampus. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 40. pp. 9124-9134.
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