Stem-like Cells in Bladder Cancer Cell Lines with Differential Sensitivity to Cisplatin

Miranda J Sarachine Falso, Bruce A. Buchholz, Ralph W deVere White

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Background: Recurrence is a common problem in bladder cancer; this has been attributed to cancer stem cells. In this study, we characterized potential cancer stem cell populations isolated from three cell lines that demonstrate different responses to cisplatin. Materials and Methods: The ALDEFLUOR® assay was used to isolate cells from TCCSUP, T24, and 5637 cell lines, and these cells were evaluated for their ability to form colonies, differentiate, migrate and invade. Results: The cell lines demonstrate a spectrum of aldehyde dehydrogenase high (ALDH High) populations that correlate with resistance to cisplatin. In the two resistant cell lines, T24 and 5637, the ALDH High cells demonstrate increased colony formation, migration, invasion, and ability to differentiate. The resistant T24 and 5637 cell lines may serve as models to investigate alternative therapies for bladder cancer.

Original languageEnglish (US)
Pages (from-to)733-738
Number of pages6
JournalAnticancer Research
Volume32
Issue number3
StatePublished - Mar 2012

Keywords

  • Aldehyde dehydrogenase
  • Bladder cancer
  • Cell lines
  • Cisplatin
  • Stem-like cells
  • T24
  • TCCSUP

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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