TY - JOUR
T1 - Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results
T2 - Metabolomic analysis of mouse tissue extracts
AU - Shellie, Robert A.
AU - Welthagen, Werner
AU - Zrostliková, Jitka
AU - Spranger, Joachim
AU - Ristow, Michael
AU - Fiehn, Oliver
AU - Zimmermann, Ralf
PY - 2005/9/9
Y1 - 2005/9/9
N2 - The potential for the utilization of GC × GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.
AB - The potential for the utilization of GC × GC-time-of-flight (TOF) MS for high-resolution metabolomics studies is discussed, with the implementation of some statistical comparisons for biomarker detection. Metabolite profiles from NZO obese mice versus BL/6 control mice are compared and contrasted using a number of chromatogram comparison routines, including direct chromatogram comparisons, chromatogram subtraction and averaging routines, as well as a method for generating relative weighted peak surface difference chromatograms, and a more conventional Student's t-test statistical approach.
KW - Data processing
KW - Gas chromatography, comprehensive two-dimensional
KW - Mass spectrometry, time-of-flight
KW - Metabolomics
UR - http://www.scopus.com/inward/record.url?scp=22744452937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22744452937&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2005.05.088
DO - 10.1016/j.chroma.2005.05.088
M3 - Article
C2 - 16130658
AN - SCOPUS:22744452937
VL - 1086
SP - 83
EP - 90
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1-2
ER -