Statistical evaluation of test accuracy studies for toxoplasma gondii in food animal intermediate hosts

Ian Gardner, M. Greiner, J. P. Dubey

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

Summary The availability of accurate diagnostic tests is essential for the detection and control of Toxoplasma gondii infections in both definitive and intermediate hosts. Sensitivity, specificity and the area under the receiver-operating characteristic (ROC) curve are commonly used measures of test accuracy for infectious diseases such as toxoplasmosis. These test performance characteristics are important considerations when selecting from among a group of tests for a specific testing purpose. In this study, we reviewed statistical approaches to evaluation of tests for toxoplasmosis with and without a gold-standard (reference) test, including use of ROC analysis and likelihood ratios which retain the diagnostic information inherent in a quantitative test result. We use previously published data from a comparison of the accuracy of serological tests for swine toxoplasmosis to demonstrate suggested methods of data analysis. We make recommendations for statistical analysis and reporting of test evaluation studies for T. gondii in food animals based on our own experiences and those of others.

Original languageEnglish (US)
Pages (from-to)82-94
Number of pages13
JournalZoonoses and Public Health
Volume57
Issue number1
DOIs
StatePublished - Feb 1 2010

Fingerprint

Toxoplasmosis
Toxoplasma
intermediate hosts
food animals
Toxoplasma gondii
Food
ROC Curve
toxoplasmosis
testing
Serologic Tests
Routine Diagnostic Tests
Gold
Communicable Diseases
Swine
Sensitivity and Specificity
animal-based foods
reference standards
immunologic techniques
diagnostic techniques
gold

Keywords

  • Diagnostic test evaluation
  • Latent class analysis
  • Likelihood ratios
  • Receiver-operating characteristic analysis
  • Test accuracy
  • Toxoplasma gondii

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Epidemiology
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Statistical evaluation of test accuracy studies for toxoplasma gondii in food animal intermediate hosts. / Gardner, Ian; Greiner, M.; Dubey, J. P.

In: Zoonoses and Public Health, Vol. 57, No. 1, 01.02.2010, p. 82-94.

Research output: Contribution to journalReview article

@article{73cef4588c624926a6be37ed6067b7e8,
title = "Statistical evaluation of test accuracy studies for toxoplasma gondii in food animal intermediate hosts",
abstract = "Summary The availability of accurate diagnostic tests is essential for the detection and control of Toxoplasma gondii infections in both definitive and intermediate hosts. Sensitivity, specificity and the area under the receiver-operating characteristic (ROC) curve are commonly used measures of test accuracy for infectious diseases such as toxoplasmosis. These test performance characteristics are important considerations when selecting from among a group of tests for a specific testing purpose. In this study, we reviewed statistical approaches to evaluation of tests for toxoplasmosis with and without a gold-standard (reference) test, including use of ROC analysis and likelihood ratios which retain the diagnostic information inherent in a quantitative test result. We use previously published data from a comparison of the accuracy of serological tests for swine toxoplasmosis to demonstrate suggested methods of data analysis. We make recommendations for statistical analysis and reporting of test evaluation studies for T. gondii in food animals based on our own experiences and those of others.",
keywords = "Diagnostic test evaluation, Latent class analysis, Likelihood ratios, Receiver-operating characteristic analysis, Test accuracy, Toxoplasma gondii",
author = "Ian Gardner and M. Greiner and Dubey, {J. P.}",
year = "2010",
month = "2",
day = "1",
doi = "10.1111/j.1863-2378.2009.01281.x",
language = "English (US)",
volume = "57",
pages = "82--94",
journal = "Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health",
issn = "1863-1959",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Statistical evaluation of test accuracy studies for toxoplasma gondii in food animal intermediate hosts

AU - Gardner, Ian

AU - Greiner, M.

AU - Dubey, J. P.

PY - 2010/2/1

Y1 - 2010/2/1

N2 - Summary The availability of accurate diagnostic tests is essential for the detection and control of Toxoplasma gondii infections in both definitive and intermediate hosts. Sensitivity, specificity and the area under the receiver-operating characteristic (ROC) curve are commonly used measures of test accuracy for infectious diseases such as toxoplasmosis. These test performance characteristics are important considerations when selecting from among a group of tests for a specific testing purpose. In this study, we reviewed statistical approaches to evaluation of tests for toxoplasmosis with and without a gold-standard (reference) test, including use of ROC analysis and likelihood ratios which retain the diagnostic information inherent in a quantitative test result. We use previously published data from a comparison of the accuracy of serological tests for swine toxoplasmosis to demonstrate suggested methods of data analysis. We make recommendations for statistical analysis and reporting of test evaluation studies for T. gondii in food animals based on our own experiences and those of others.

AB - Summary The availability of accurate diagnostic tests is essential for the detection and control of Toxoplasma gondii infections in both definitive and intermediate hosts. Sensitivity, specificity and the area under the receiver-operating characteristic (ROC) curve are commonly used measures of test accuracy for infectious diseases such as toxoplasmosis. These test performance characteristics are important considerations when selecting from among a group of tests for a specific testing purpose. In this study, we reviewed statistical approaches to evaluation of tests for toxoplasmosis with and without a gold-standard (reference) test, including use of ROC analysis and likelihood ratios which retain the diagnostic information inherent in a quantitative test result. We use previously published data from a comparison of the accuracy of serological tests for swine toxoplasmosis to demonstrate suggested methods of data analysis. We make recommendations for statistical analysis and reporting of test evaluation studies for T. gondii in food animals based on our own experiences and those of others.

KW - Diagnostic test evaluation

KW - Latent class analysis

KW - Likelihood ratios

KW - Receiver-operating characteristic analysis

KW - Test accuracy

KW - Toxoplasma gondii

UR - http://www.scopus.com/inward/record.url?scp=74049123096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74049123096&partnerID=8YFLogxK

U2 - 10.1111/j.1863-2378.2009.01281.x

DO - 10.1111/j.1863-2378.2009.01281.x

M3 - Review article

C2 - 19744298

AN - SCOPUS:74049123096

VL - 57

SP - 82

EP - 94

JO - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

JF - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

SN - 1863-1959

IS - 1

ER -