Statistical design of ELISA protocols

David S. Bunch, David M Rocke, Robert O. Harrison

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.

Original languageEnglish (US)
Pages (from-to)247-254
Number of pages8
JournalJournal of Immunological Methods
Volume132
Issue number2
DOIs
StatePublished - Sep 14 1990

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Calibration
Enzyme-Linked Immunosorbent Assay

Keywords

  • Immunoassay
  • Microplate 96-well
  • Optimal design

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Statistical design of ELISA protocols. / Bunch, David S.; Rocke, David M; Harrison, Robert O.

In: Journal of Immunological Methods, Vol. 132, No. 2, 14.09.1990, p. 247-254.

Research output: Contribution to journalArticle

Bunch, David S. ; Rocke, David M ; Harrison, Robert O. / Statistical design of ELISA protocols. In: Journal of Immunological Methods. 1990 ; Vol. 132, No. 2. pp. 247-254.
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