Statistical design of ELISA protocols

David S. Bunch, David M Rocke, Robert O. Harrison

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.

Original languageEnglish (US)
Pages (from-to)247-254
Number of pages8
JournalJournal of Immunological Methods
Issue number2
StatePublished - Sep 14 1990


  • Immunoassay
  • Microplate 96-well
  • Optimal design

ASJC Scopus subject areas

  • Biotechnology
  • Immunology


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