Standing genetic variation in contingency loci drives the rapid adaptation of Campylobacter jejuni to a novel host

John P. Jerome, Julia A. Bell, Anne E. Plovanich-Jones, Jeffrey E. Barrick, Charles Brown, Linda S. Mansfield

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The genome of the food-borne pathogen Campylobacter jejuni contains multiple highly mutable sites, or contingency loci. It has been suggested that standing variation at these loci is a mechanism for rapid adaptation to a novel environment, but this phenomenon has not been shown experimentally. In previous work we showed that the virulence of C. jejuni NCTC11168 increased after serial passage through a C57BL/6 IL-10-/- mouse model of campylobacteriosis. Here we sought to determine the genetic basis of this adaptation during passage. Re-sequencing of the 1.64Mb genome to 200-500X coverage allowed us to define variation in 23 contingency loci to an unprecedented depth both before and after in vivo adaptation. Mutations in the mouse-adapted C. jejuni were largely restricted to the homopolymeric tracts of thirteen contingency loci. These changes cause significant alterations in open reading frames of genes in surface structure biosynthesis loci and in genes with only putative functions. Several loci with open reading frame changes also had altered transcript abundance. The increase in specific phases of contingency loci during in vivo passage of C. jejuni, coupled with the observed virulence increase and the lack of other types of genetic changes, is the first experimental evidence that these variable regions play a significant role in C. jejuni adaptation and virulence in a novel host.

Original languageEnglish (US)
Article numbere16399
JournalPLoS One
Volume6
Issue number1
DOIs
StatePublished - Feb 7 2011
Externally publishedYes

Fingerprint

Campylobacter jejuni
Genes
genetic variation
loci
Virulence
Open Reading Frames
virulence
Genome
Biosynthesis
Serial Passage
Pathogens
open reading frames
Surface structure
Interleukin-10
campylobacteriosis
genome
food pathogens
interleukin-10
Food
Mutation

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Standing genetic variation in contingency loci drives the rapid adaptation of Campylobacter jejuni to a novel host. / Jerome, John P.; Bell, Julia A.; Plovanich-Jones, Anne E.; Barrick, Jeffrey E.; Brown, Charles; Mansfield, Linda S.

In: PLoS One, Vol. 6, No. 1, e16399, 07.02.2011.

Research output: Contribution to journalArticle

Jerome, John P. ; Bell, Julia A. ; Plovanich-Jones, Anne E. ; Barrick, Jeffrey E. ; Brown, Charles ; Mansfield, Linda S. / Standing genetic variation in contingency loci drives the rapid adaptation of Campylobacter jejuni to a novel host. In: PLoS One. 2011 ; Vol. 6, No. 1.
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