Stain-Free total protein staining is a superior loading control to b-actin for Western blots

Jennifer E. Gilda, Aldrin V Gomes

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

Semi-quantification of proteins using Western blots typically involves normalization against housekeeping genes such as b-actin. More recently, Ponceau S and Coomassie blue staining have both been shown to be suitable alternatives to housekeeping genes as loading controls. Stain-Free total protein staining offers the advantage of no staining or destaining steps. Evaluation of the use of Stain-Free staining as an alternative to b-actin or the protein stain Ponceau S showed that Stain-Free staining was superior to b-actin and as good as or better than Ponceau S staining as a loading control for Western blots.

Original languageEnglish (US)
Pages (from-to)186-188
Number of pages3
JournalAnalytical Biochemistry
Volume440
Issue number2
DOIs
StatePublished - 2013

Keywords

  • B-Actin
  • Nitrocellulose
  • Ponceau S
  • Stain Free
  • Western blotting

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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