Stable association between strains of Mycobacterium tuberculosis and their human host populations

Aaron E. Hirsh, Anthony G. Tsolaki, Kathryn DeRiemer, Marcus W. Feldman, Peter M. Small

Research output: Contribution to journalArticle

273 Citations (Scopus)

Abstract

Mycobacterium tuberculosis is an important human pathogen in virtually every part of the world. Here we investigate whether distinct strains of M. tuberculosis infect different human populations and whether associations between host and pathogen populations are stable despite global traffic and the convergence of diverse strains of the pathogen in cosmopolitan urban centers. The recent global movement and transmission history of 100 M. tuberculosis isolates was inferred from a molecular epidemiologic study of tuberculosis that spans 12 years. Genetic relationships among these isolates were deduced from the distribution of large genomic deletions, which were identified by DNA microarray and confirmed by PCR and sequence analysis. Phylogenetic analysis of these deletions indicates that they are unique event polymorphisms and that horizontal gene transfer is extremely rare in M. tuberculosis. In conjunction with the epidemiological data, phylogenies reveal three large phylogeographic regions. A host's region of origin is predictive of the strain of tuberculosis he or she carries, and this association remains strong even when transmission takes place in a cosmopolitan urban center outside of the region of origin. Approximate dating of the time since divergence of East Asian and Philippine clades of M. tuberculosis suggests that these lineages diverged centuries ago. Thus, associations between host and pathogen populations appear to be highly stable.

Original languageEnglish (US)
Pages (from-to)4871-4876
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number14
DOIs
StatePublished - Apr 6 2004
Externally publishedYes

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Mycobacterium tuberculosis
Population
Tuberculosis
Horizontal Gene Transfer
Philippines
Population Dynamics
Phylogeny
Oligonucleotide Array Sequence Analysis
Sequence Analysis
Epidemiologic Studies
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Stable association between strains of Mycobacterium tuberculosis and their human host populations. / Hirsh, Aaron E.; Tsolaki, Anthony G.; DeRiemer, Kathryn; Feldman, Marcus W.; Small, Peter M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 14, 06.04.2004, p. 4871-4876.

Research output: Contribution to journalArticle

Hirsh, Aaron E. ; Tsolaki, Anthony G. ; DeRiemer, Kathryn ; Feldman, Marcus W. ; Small, Peter M. / Stable association between strains of Mycobacterium tuberculosis and their human host populations. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 14. pp. 4871-4876.
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