Stabilization of membranes in human platelets freeze-dried with trehalose

John H. Crowe, Fern Tablin, Willem F. Wolkers, Karine Gousset, Nelly M. Tsvetkova, Josette Ricker

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Human blood platelets are normally stored in blood banks for 3-5 days, after which they are discarded. We have launched an effort at developing means for preserving the platelets for long term storage. In previous studies we have shown that trehalose can be used to preserve biological membranes and proteins during drying and have provided evidence concerning the mechanism. A myth has grown up about special properties of trehalose, which we discuss here and clarify some of what is fact and what is misconception. We have found a simple way of introducing this sugar into the cytoplasm of platelets and have successfully freeze-dried the trehalose-loaded platelets, with very promising results. We present evidence that membrane microdomains are maintained intact in the platelets freeze-dried with trehalose. Finally, we propose a possible mechanism by which the microdomains are preserved.

Original languageEnglish (US)
Pages (from-to)41-52
Number of pages12
JournalChemistry and Physics of Lipids
Volume122
Issue number1-2
DOIs
StatePublished - Jan 2003

Fingerprint

Trehalose
Platelets
Blood Platelets
Stabilization
Membranes
Blood
Membrane Microdomains
Biological membranes
Blood Banks
Sugars
Drying
Membrane Proteins
Cytoplasm

Keywords

  • Freeze-dried
  • Human platelets
  • Trehalose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Stabilization of membranes in human platelets freeze-dried with trehalose. / Crowe, John H.; Tablin, Fern; Wolkers, Willem F.; Gousset, Karine; Tsvetkova, Nelly M.; Ricker, Josette.

In: Chemistry and Physics of Lipids, Vol. 122, No. 1-2, 01.2003, p. 41-52.

Research output: Contribution to journalArticle

Crowe, John H. ; Tablin, Fern ; Wolkers, Willem F. ; Gousset, Karine ; Tsvetkova, Nelly M. ; Ricker, Josette. / Stabilization of membranes in human platelets freeze-dried with trehalose. In: Chemistry and Physics of Lipids. 2003 ; Vol. 122, No. 1-2. pp. 41-52.
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