Stabilization of bovine trypsin by reductive methylation

Robert H. Rice, Gary E. Means, W. Duane Brown

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Reductive methylation has little or no detectable effect on the catalytic or physicochemical properties of bovine trypsin but reduces its susceptibility to autolysis. Increased stability after methylation appears to result from the conversion of trypsin-susceptible lysine residues to trypsin-resistant ε-N,N-dimethyllysine residues. Reductively methylated trypsin is easily prepared and may be useful in place of trypsin where autolysis is otherwise difficult to control.

Original languageEnglish (US)
Pages (from-to)316-321
Number of pages6
JournalBBA - Protein Structure
Volume492
Issue number2
DOIs
StatePublished - Jun 24 1977

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Methylation
Trypsin
Stabilization
Autolysis
Lysine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Stabilization of bovine trypsin by reductive methylation. / Rice, Robert H.; Means, Gary E.; Brown, W. Duane.

In: BBA - Protein Structure, Vol. 492, No. 2, 24.06.1977, p. 316-321.

Research output: Contribution to journalArticle

Rice, Robert H. ; Means, Gary E. ; Brown, W. Duane. / Stabilization of bovine trypsin by reductive methylation. In: BBA - Protein Structure. 1977 ; Vol. 492, No. 2. pp. 316-321.
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