Stabilization of 8S progesterone receptor from human prostate in the presence of molybdate ion

Charles L Bevins, N. Bashirelahi

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

In the presence of 10 mM molybdate ion, the authors were able to detect the appearance of a [6,7-3H]-17,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione-([3H]R5020) binding moiety in human prostatic cytosol which sedimented at approximately 8S in a glycerol density gradient. The specifically bound [3H]R5020 was displaced by progesterone, triamcinolone acetonide, and R5020 but not by cortisol, dihydrotestosterone, 17β-estradiol, or diethylstilbestrol. Specific binding of [3H]R5020 in the presence of molybdate ion was shown to saturate at 7 x 10-10 M (n = 64 fmol/mg of protein). The optimum concentration of molybdate ion for enhancing specific binding of [3H]R5020 was determined to be between 7 and 20 mM. Molybdate ion was also effective in stabilizing the 8S R5020-binding moiety in a preincubation for 16 hr at 0° in the absence of added steroid.

Original languageEnglish (US)
Pages (from-to)2234-2239
Number of pages6
JournalCancer Research
Volume40
Issue number7
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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