Stabilization and electrophoretic analysis of meiotic recombination intermediates in Saccharomyces cerevisiae

Steve D. Oh, Lea Jessop, Jessica P. Lao, Thorsten Allers, Michael Lichten, Neil Hunter

Research output: Chapter in Book/Report/Conference proceedingChapter

36 Scopus citations

Abstract

Joint Molecule (JM) recombination intermediates result from DNA strand-exchange between homologous chromosomes. Physical monitoring of JM formation in budding yeast has provided a wealth of information about the timing and mechanism of meiotic recombination. These assays are especially informative when applied to the analysis of mutants for which genetic analysis of recombination is impossible, i.e. mutants that die during meiosis. This chapter describes three distinct methods to stabilize JMs against thermally driven dissolution as well as electrophoretic approaches to resolve and detect JMs at two well-characterized recombination hotspots.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages209-234
Number of pages26
Volume557
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume557
ISSN (Print)10643745

Keywords

  • D-loop
  • double-strand break
  • electrophoresis
  • Holliday junction
  • homologous recombination
  • joint molecule
  • Meiosis
  • strand-exchange

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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