Abstract
CD133+ populations of human glioblastoma multiforme (GBM) cells are reportedly enriched for tumor stem cells (TSCs) or tumor-initiating cells (TICs). Approximately 40% of freshly isolated GBM specimens, however, do not contain CD133+ tumor cells, raising the possibility that CD133 may not be a universal enrichment marker for GBM TSCs/TICs. Here we demonstrate that stage-specific embryonic antigen 1(SSEA-1/LeX)+ GBM cells fulfill the functional criteria for TSC/TIC, since (1) SSEA-1+ cells are highly tumorigenic in vivo, unlike SSEA-1- cells; (2) SSEA-1+ cells can give rise to both SSEA-1+ and SSEA-1- cells, thereby establishing a cellular hierarchy; and (3) SSEA-1+ cells have self-renewal and multilineage differentiation potentials. A distinct subpopulation of SSEA-1+ cells was present in all but one of the primary GBMs examined (n = 24), and most CD133+ tumor cells were also SSEA-1+, suggesting that SSEA-1 may be a general TSC/TIC enrichment marker in human GBMs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 440-452 |
| Number of pages | 13 |
| Journal | Cell Stem Cell |
| Volume | 4 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 8 2009 |
| Externally published | Yes |
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Keywords
- STEMCELL
ASJC Scopus subject areas
- Cell Biology
- Molecular Medicine
- Genetics
Cite this
SSEA-1 Is an Enrichment Marker for Tumor-Initiating Cells in Human Glioblastoma. / Son, Myung Jin; Woolard, Kevin D; Nam, Do Hyun; Lee, Jeongwu; Fine, Howard A.
In: Cell Stem Cell, Vol. 4, No. 5, 08.05.2009, p. 440-452.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SSEA-1 Is an Enrichment Marker for Tumor-Initiating Cells in Human Glioblastoma
AU - Son, Myung Jin
AU - Woolard, Kevin D
AU - Nam, Do Hyun
AU - Lee, Jeongwu
AU - Fine, Howard A.
PY - 2009/5/8
Y1 - 2009/5/8
N2 - CD133+ populations of human glioblastoma multiforme (GBM) cells are reportedly enriched for tumor stem cells (TSCs) or tumor-initiating cells (TICs). Approximately 40% of freshly isolated GBM specimens, however, do not contain CD133+ tumor cells, raising the possibility that CD133 may not be a universal enrichment marker for GBM TSCs/TICs. Here we demonstrate that stage-specific embryonic antigen 1(SSEA-1/LeX)+ GBM cells fulfill the functional criteria for TSC/TIC, since (1) SSEA-1+ cells are highly tumorigenic in vivo, unlike SSEA-1- cells; (2) SSEA-1+ cells can give rise to both SSEA-1+ and SSEA-1- cells, thereby establishing a cellular hierarchy; and (3) SSEA-1+ cells have self-renewal and multilineage differentiation potentials. A distinct subpopulation of SSEA-1+ cells was present in all but one of the primary GBMs examined (n = 24), and most CD133+ tumor cells were also SSEA-1+, suggesting that SSEA-1 may be a general TSC/TIC enrichment marker in human GBMs.
AB - CD133+ populations of human glioblastoma multiforme (GBM) cells are reportedly enriched for tumor stem cells (TSCs) or tumor-initiating cells (TICs). Approximately 40% of freshly isolated GBM specimens, however, do not contain CD133+ tumor cells, raising the possibility that CD133 may not be a universal enrichment marker for GBM TSCs/TICs. Here we demonstrate that stage-specific embryonic antigen 1(SSEA-1/LeX)+ GBM cells fulfill the functional criteria for TSC/TIC, since (1) SSEA-1+ cells are highly tumorigenic in vivo, unlike SSEA-1- cells; (2) SSEA-1+ cells can give rise to both SSEA-1+ and SSEA-1- cells, thereby establishing a cellular hierarchy; and (3) SSEA-1+ cells have self-renewal and multilineage differentiation potentials. A distinct subpopulation of SSEA-1+ cells was present in all but one of the primary GBMs examined (n = 24), and most CD133+ tumor cells were also SSEA-1+, suggesting that SSEA-1 may be a general TSC/TIC enrichment marker in human GBMs.
KW - STEMCELL
UR - http://www.scopus.com/inward/record.url?scp=65349115320&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349115320&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2009.03.003
DO - 10.1016/j.stem.2009.03.003
M3 - Article
C2 - 19427293
AN - SCOPUS:65349115320
VL - 4
SP - 440
EP - 452
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 5
ER -