SSCP analysis of scnDNA for genetic profiling of Aedes aegypti

Jacklyn Wong, Frédéric Tripet, Jason L. Rasgon, Gregory C Lanzaro, Thomas W. Scott

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume79
Issue number4
StatePublished - Oct 2008

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

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