Spontaneous TH1 cytokine production by intraepithelial but not circulating T cells in infants with or without food allergies

M. A. Pérez-Machado, Paul Ashwood, F. Torrente, C. Salvestrini, R. Sim, M. A. Thomson, J. A. Walker-Smith, S. H. Murch

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: It has been established that the maintenance of immunological tolerance to dietary antigen and the intestinal flora (oral tolerance) is an actively-maintained process dependent upon mucosal lymphocyte populations. Early life exposures appear critical in the development of such tolerance. However little is known about the activation status of mucosal lymphocytes in human infancy and childhood. Patients and methods: We have performed flow cytometric analysis for cell lineage and cytokine-production status in peripheral blood and duodenal intraepithelial lymphocytes taken during endoscopy from 20 children [median age 2.9 ± 0.6 years (median ± SE)] in whom investigation found no intestinal abnormalities (histologically normal controls) and 30 children (median age 1.6 ± 0.4 years) with confirmed allergy to cow's milk and other dietary antigens. Results: Regardless of clinical status, spontaneous production of cytokines was low or undetectable in peripheral blood cells. By contrast, intraepithelial CD4 and CD8 cells isolated from the small intestine were often activated, with 5% or more showing spontaneous production of TH1 type [interleukin-2, interferon (IFN)-γ] cytokines in both normal controls and food-allergic children. Stimulation in vitro strongly induced cytokine production in peripheral blood but not intraepithelial lymphocytes. Immunohistochemistry showed similar density of IFN-γ+ intraepithelial lymphocytes in controls and allergic children. Conclusions: Duodenal intraepithelial lymphocytes in human infants show a state of increased spontaneous activation compared with peripheral blood lymphocytes, and show no significant impairment of T H1 responses in food allergic children.

Original languageEnglish (US)
Pages (from-to)346-353
Number of pages8
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume59
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Food Hypersensitivity
Lymphocytes
Cytokines
T-Lymphocytes
Antigens
Food
Interleukins
Cell Lineage
Interferons
Endoscopy
Interferon-gamma
Small Intestine
Blood Cells
Hypersensitivity
Milk
Immunohistochemistry
Maintenance
Population

Keywords

  • Cytokines
  • Food allergy
  • T cells

ASJC Scopus subject areas

  • Immunology

Cite this

Spontaneous TH1 cytokine production by intraepithelial but not circulating T cells in infants with or without food allergies. / Pérez-Machado, M. A.; Ashwood, Paul; Torrente, F.; Salvestrini, C.; Sim, R.; Thomson, M. A.; Walker-Smith, J. A.; Murch, S. H.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 59, No. 3, 03.2004, p. 346-353.

Research output: Contribution to journalArticle

Pérez-Machado, M. A. ; Ashwood, Paul ; Torrente, F. ; Salvestrini, C. ; Sim, R. ; Thomson, M. A. ; Walker-Smith, J. A. ; Murch, S. H. / Spontaneous TH1 cytokine production by intraepithelial but not circulating T cells in infants with or without food allergies. In: Allergy: European Journal of Allergy and Clinical Immunology. 2004 ; Vol. 59, No. 3. pp. 346-353.
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AU - Salvestrini, C.

AU - Sim, R.

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