Spontaneous Calcium Waves without Contraction in Cardiac Myocytes

J. R. Lopez, A. Jovanovic, A. Terzic

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.

Original languageEnglish (US)
Pages (from-to)781-787
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number3
DOIs
StatePublished - Sep 25 1995
Externally publishedYes

Fingerprint

Calcium Signaling
Cardiac Myocytes
Calcium
Confocal Microscopy
Wave propagation
Sarcomeres
Fluorescent Dyes
Muscle Cells
Confocal microscopy
Lasers

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Spontaneous Calcium Waves without Contraction in Cardiac Myocytes. / Lopez, J. R.; Jovanovic, A.; Terzic, A.

In: Biochemical and Biophysical Research Communications, Vol. 214, No. 3, 25.09.1995, p. 781-787.

Research output: Contribution to journalArticle

Lopez, J. R. ; Jovanovic, A. ; Terzic, A. / Spontaneous Calcium Waves without Contraction in Cardiac Myocytes. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 214, No. 3. pp. 781-787.
@article{448d98442c634653a0442d1f45e03969,
title = "Spontaneous Calcium Waves without Contraction in Cardiac Myocytes",
abstract = "Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.",
author = "Lopez, {J. R.} and A. Jovanovic and A. Terzic",
year = "1995",
month = "9",
day = "25",
doi = "10.1006/bbrc.1995.2354",
language = "English (US)",
volume = "214",
pages = "781--787",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Spontaneous Calcium Waves without Contraction in Cardiac Myocytes

AU - Lopez, J. R.

AU - Jovanovic, A.

AU - Terzic, A.

PY - 1995/9/25

Y1 - 1995/9/25

N2 - Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.

AB - Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.

UR - http://www.scopus.com/inward/record.url?scp=0028866206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028866206&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.2354

DO - 10.1006/bbrc.1995.2354

M3 - Article

C2 - 7575544

AN - SCOPUS:0028866206

VL - 214

SP - 781

EP - 787

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -