Abstract
Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 781-787 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 214 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 25 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Medicine(all)
- Molecular Biology
- Cell Biology
Cite this
Spontaneous Calcium Waves without Contraction in Cardiac Myocytes. / Lopez, J. R.; Jovanovic, A.; Terzic, A.
In: Biochemical and Biophysical Research Communications, Vol. 214, No. 3, 25.09.1995, p. 781-787.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spontaneous Calcium Waves without Contraction in Cardiac Myocytes
AU - Lopez, J. R.
AU - Jovanovic, A.
AU - Terzic, A.
PY - 1995/9/25
Y1 - 1995/9/25
N2 - Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.
AB - Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028866206&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.2354
DO - 10.1006/bbrc.1995.2354
M3 - Article
C2 - 7575544
AN - SCOPUS:0028866206
VL - 214
SP - 781
EP - 787
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -