Abstract
Spontaneous Ca2+ waves were visualized in quiescent cardiomyocytes loaded with the Ca2+-sensitive fluorescent probe, Fluo-3, and imaged by laser confocal microscopy. No sarcomere shortening was detected during wave propagation. This type of Ca2+ waves began at the periphery or in a central region of a myocyte and propagated the length of the cell in one or two directions. The average velocity of wave propagation was 32 μm/sec and the estimated concentration of Ca2+ oscillated from 124, at the bottom, to 311 nM, at the pick of the wave. Ca2+ waves were not confined to a single cell but could spread from cell to cell. These results describe a type of spontaneous Ca2+ waves which does not induce a contractile response in cardiomyocytes.
Original language | English (US) |
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Pages (from-to) | 781-787 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 214 |
Issue number | 3 |
DOIs | |
State | Published - Sep 25 1995 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Medicine(all)
- Molecular Biology
- Cell Biology