Spontaneous and double-strand break-induced recombination, and gene conversion tract lengths, are differentially affected by overexpression of wild-type or ATPase-defective yeast Rad54

Perry M. Kim, Kimberly S. Paffett, Jachen A. Solinger, Wolf Dietrich Heyer, Jac A. Nickoloff

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Rad54 plays key roles in homologous recombination (HR) and double-strand break (DSB) repair in yeast, along with Rad51, Rad52, Rad55 and Rad57. Rad54 belongs to the Swi2/Snf2 family of DNA-stimulated ATPases. Rad51 nucleoprotein filaments catalyze DNA strand exchange and Rad54 augments this activity of Rad51. Mutations in the Rad54 ATPase domain (ATPase-) impair Rad54 function in vitro, sensitize yeast to killing by methylmethane sulfonate and reduce spontaneous gene conversion. We found that overexpression of ATPase- Rad54 reduced spontaneous direct repeat gene conversion and increased both spontaneous direct repeat deletion and spontaneous allelic conversion. Overexpression of ATPase- Rad54 decreased DSB-induced allelic conversion, but increased chromosome loss and DSB-dependent lethality. Thus, ATP hydrolysis by Rad54 contributes to genome stability by promoting high-fidelity DSB repair and suppressing spontaneous deletions. Overexpression of wild-type Rad54 did not alter DSB-induced HR levels, but conversion tract lengths were reduced. Interestingly, ATPase- Rad54 decreased overall HR levels and increased tract lengths. These tract length changes provide new in vivo evidence that Rad54 functions in the post-synaptic phase during recombinational repair of DSBs.

Original languageEnglish (US)
Pages (from-to)2727-2735
Number of pages9
JournalNucleic Acids Research
Volume30
Issue number13
StatePublished - Jul 1 2002

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Gene Conversion
Genetic Recombination
Adenosine Triphosphatases
Yeasts
Homologous Recombination
Nucleic Acid Repetitive Sequences
Methyl Methanesulfonate
Nucleoproteins
Genomic Instability
DNA
Hydrolysis
Chromosomes
Adenosine Triphosphate
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

Spontaneous and double-strand break-induced recombination, and gene conversion tract lengths, are differentially affected by overexpression of wild-type or ATPase-defective yeast Rad54. / Kim, Perry M.; Paffett, Kimberly S.; Solinger, Jachen A.; Heyer, Wolf Dietrich; Nickoloff, Jac A.

In: Nucleic Acids Research, Vol. 30, No. 13, 01.07.2002, p. 2727-2735.

Research output: Contribution to journalArticle

Kim, Perry M. ; Paffett, Kimberly S. ; Solinger, Jachen A. ; Heyer, Wolf Dietrich ; Nickoloff, Jac A. / Spontaneous and double-strand break-induced recombination, and gene conversion tract lengths, are differentially affected by overexpression of wild-type or ATPase-defective yeast Rad54. In: Nucleic Acids Research. 2002 ; Vol. 30, No. 13. pp. 2727-2735.
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