Splicing of a yeast proline tRNA containing a novel suppressor mutation in the anticodon stem

Mark Winey, Michael D. Mendenhall, Claudia M. Cummins, Michael R. Culbertson, Gayle Knapp

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The intron-containing proline tRNAUGG genes in Saccharomyces cerevisiae can mutate to suppress +1 frameshift mutations in proline codons via a G to U base substitution mutation at position 39. The mutation alters the 3′ splice junction and disrupts the bottom base-pair of the anticodon stem which presumably allows the tRNA to read a four-base codon. In order to understand the mechanism of suppression and to study the splicing of suppressor pre-tRNA, we determined the sequences of the mature wild-type and mutant suppressor gene products in vivo and analyzed splicing of the corresponding pre-tRNAs in vitro. We show that a novel tRNA isolated from suppressor strains is the product of frameshift suppressor genes. Sequence analysis indicated that suppressor pre-tRNA is spliced at the same sites as wild-type pre-tRNA. The tRNA therefore contains a four-base anticodon stem and nine-base anticodon loop. Analysis of suppressor pre-tRNA in vitro revealed that endonuclease cleavage at the 3′ splice junction occurred with reduced efficiency compared to wild-type. In addition, reduced accumulation of mature suppressor tRNA was observed in a combined cleavage and ligation reaction. These results suggest that cleavage at the 3′ splice junction is inefficient but not abolished. The novel tRNA from suppressor strains was shown to be the functional agent of suppression by deleting the intron from a suppressor gene. The tRNA produced in vivo from this gene is identical to that of the product of an intron+ gene, indicating that the intron is not required for proper base modification. The product of the intron- gene is a more efficient suppressor than the product of an intron+ gene. One interpretation of this result is that inefficient splicing in vivo may be limiting the steady-state level of mature suppressor tRNA.

Original languageEnglish (US)
Pages (from-to)49-63
Number of pages15
JournalJournal of Molecular Biology
Volume192
Issue number1
DOIs
StatePublished - Nov 5 1986
Externally publishedYes

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Genetic Suppression
Anticodon
Transfer RNA
Proline
Introns
RNA Precursors
Yeasts
Suppressor Genes
Genes
Codon
Frameshift Mutation
Mutation
Endonucleases
Base Pairing
Ligation
Sequence Analysis
Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Splicing of a yeast proline tRNA containing a novel suppressor mutation in the anticodon stem. / Winey, Mark; Mendenhall, Michael D.; Cummins, Claudia M.; Culbertson, Michael R.; Knapp, Gayle.

In: Journal of Molecular Biology, Vol. 192, No. 1, 05.11.1986, p. 49-63.

Research output: Contribution to journalArticle

Winey, Mark ; Mendenhall, Michael D. ; Cummins, Claudia M. ; Culbertson, Michael R. ; Knapp, Gayle. / Splicing of a yeast proline tRNA containing a novel suppressor mutation in the anticodon stem. In: Journal of Molecular Biology. 1986 ; Vol. 192, No. 1. pp. 49-63.
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