Splice variants of PAC1 receptor during early neural development of rats

Chengji Zhou, Sakae Kikuyama, Shigeo Nakajo, Takahiro Hirabayashi, Hidekatsu Mizushima, Seiji Shioda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)1177-1183
Number of pages7
JournalPeptides
Volume21
Issue number8
DOIs
StatePublished - Aug 2000
Externally publishedYes

Fingerprint

Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Humulus
Rats
Protein Isoforms
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Primitive Streak
Signal transduction
Polymerase chain reaction
Alternative Splicing
Transcription
Reverse Transcription
Hip
Signal Transduction
Polymerase Chain Reaction

Keywords

  • G-protein coupled receptor
  • In situ RT- PCR
  • Neuroepithelia
  • PACAP
  • Rat
  • Splice variants

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Zhou, C., Kikuyama, S., Nakajo, S., Hirabayashi, T., Mizushima, H., & Shioda, S. (2000). Splice variants of PAC1 receptor during early neural development of rats. Peptides, 21(8), 1177-1183. https://doi.org/10.1016/S0196-9781(00)00257-6

Splice variants of PAC1 receptor during early neural development of rats. / Zhou, Chengji; Kikuyama, Sakae; Nakajo, Shigeo; Hirabayashi, Takahiro; Mizushima, Hidekatsu; Shioda, Seiji.

In: Peptides, Vol. 21, No. 8, 08.2000, p. 1177-1183.

Research output: Contribution to journalArticle

Zhou, C, Kikuyama, S, Nakajo, S, Hirabayashi, T, Mizushima, H & Shioda, S 2000, 'Splice variants of PAC1 receptor during early neural development of rats', Peptides, vol. 21, no. 8, pp. 1177-1183. https://doi.org/10.1016/S0196-9781(00)00257-6
Zhou C, Kikuyama S, Nakajo S, Hirabayashi T, Mizushima H, Shioda S. Splice variants of PAC1 receptor during early neural development of rats. Peptides. 2000 Aug;21(8):1177-1183. https://doi.org/10.1016/S0196-9781(00)00257-6
Zhou, Chengji ; Kikuyama, Sakae ; Nakajo, Shigeo ; Hirabayashi, Takahiro ; Mizushima, Hidekatsu ; Shioda, Seiji. / Splice variants of PAC1 receptor during early neural development of rats. In: Peptides. 2000 ; Vol. 21, No. 8. pp. 1177-1183.
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