Abstract
The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1177-1183 |
| Number of pages | 7 |
| Journal | Peptides |
| Volume | 21 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2000 |
| Externally published | Yes |
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Keywords
- G-protein coupled receptor
- In situ RT- PCR
- Neuroepithelia
- PACAP
- Rat
- Splice variants
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
Splice variants of PAC1 receptor during early neural development of rats. / Zhou, Chengji; Kikuyama, Sakae; Nakajo, Shigeo; Hirabayashi, Takahiro; Mizushima, Hidekatsu; Shioda, Seiji.
In: Peptides, Vol. 21, No. 8, 08.2000, p. 1177-1183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Splice variants of PAC1 receptor during early neural development of rats
AU - Zhou, Chengji
AU - Kikuyama, Sakae
AU - Nakajo, Shigeo
AU - Hirabayashi, Takahiro
AU - Mizushima, Hidekatsu
AU - Shioda, Seiji
PY - 2000/8
Y1 - 2000/8
N2 - The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.
AB - The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.
KW - G-protein coupled receptor
KW - In situ RT- PCR
KW - Neuroepithelia
KW - PACAP
KW - Rat
KW - Splice variants
UR - http://www.scopus.com/inward/record.url?scp=0033782302&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033782302&partnerID=8YFLogxK
U2 - 10.1016/S0196-9781(00)00257-6
DO - 10.1016/S0196-9781(00)00257-6
M3 - Article
C2 - 11035203
AN - SCOPUS:0033782302
VL - 21
SP - 1177
EP - 1183
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 8
ER -