Splice variants of PAC1 receptor during early neural development of rats

Chengji Zhou; Sakae Kikuyama; Shigeo Nakajo; Takahiro Hirabayashi; Hidekatsu Mizushima; Seiji Shioda

doi:10.1016/S0196-9781(00)00257-6

Splice variants of PAC₁ receptor during early neural development of rats

Chengji Zhou, Sakae Kikuyama, Shigeo Nakajo, Takahiro Hirabayashi, Hidekatsu Mizushima, Seiji Shioda

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC₁-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC₁ receptor seen during development are the short splice isoform, PAC₁-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC₁-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	1177-1183
Number of pages	7
Journal	Peptides
Volume	21
Issue number	8
DOIs	https://doi.org/10.1016/S0196-9781(00)00257-6
State	Published - Aug 2000
Externally published	Yes

Keywords

G-protein coupled receptor
In situ RT- PCR
Neuroepithelia
PACAP
Rat
Splice variants

ASJC Scopus subject areas

Biochemistry
Endocrinology
Physiology
Cellular and Molecular Neuroscience

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10.1016/S0196-9781(00)00257-6

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Cite this

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title = "Splice variants of PAC1 receptor during early neural development of rats",

abstract = "The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.",

keywords = "G-protein coupled receptor, In situ RT- PCR, Neuroepithelia, PACAP, Rat, Splice variants",

author = "Chengji Zhou and Sakae Kikuyama and Shigeo Nakajo and Takahiro Hirabayashi and Hidekatsu Mizushima and Seiji Shioda",

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AU - Zhou, Chengji

AU - Kikuyama, Sakae

AU - Nakajo, Shigeo

AU - Hirabayashi, Takahiro

AU - Mizushima, Hidekatsu

AU - Shioda, Seiji

PY - 2000/8

Y1 - 2000/8

N2 - The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.

AB - The specific pituitary adenylate cyclase-activating polypeptide (PACAP) receptor, PAC1-R, consists of at least seven isoforms, and they are differentially coupled to signal transduction pathways by alternative splicing. We have found that the major splice variants of the PAC1 receptor seen during development are the short splice isoform, PAC1-R-s (which does not contain either the 'hip' or 'hop' cassette), and another form, PAC1-R- hop (which contains the 'hop' cassette). We also have applied an innovative molecular histochemical technique, in situ reverse transcription-polymerase chain reaction (RT-PCR), and determined that these two splice isoforms are colocalized in the neuroepithelia from the primitive streak stage. (C) 2000 Elsevier Science Inc.

KW - G-protein coupled receptor

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KW - PACAP

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KW - Splice variants

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