Spleen hemostasis using high-intensity ultrasound: Survival and healing

Misty L. Noble, Shahram Vaezy, Amid Keshavarzi, Marla Paun, Adrian F. Prokop, Emil Y. Chi, Carol Cornejo, Sam R. Sharar, Gregory Jurkovich, Roy W. Martin, Lawrence A. Crum

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Background: Previous studies have shown that high-intensity focused ultrasound (HIFU) can effectively control bleeding of incised livers and spleens and punctured vessels. This current study investigated the long-term safety of HIFU in splenic hemostasis. Methods: A total of 21 rabbits were randomly assigned to two groups: HIFU treatment (n = 14), and sham treatment (n = 7). All animals underwent sterile laparotomy and splenic exposure. The HIFU-treated animals received splenic incisions, 8 to 10 mm long and 4 to 5 mm deep, and immediate 9.6-MHz HIFU until hemostasis was achieved. After recovery, ultrasound images, blood samples, and histologic samples were collected on days 0, 1, 3, 7, 14, 28, and 60. Results: All 14 splenic injuries were hemostatic after an average of 96 seconds of HIFU application. There was evidence of rebleeding in one animal between days 3 and 7 posttreatment. Subsequent blood analysis showed no significant difference in serial hematologic or coagulation measures between HIFU and sham groups. Histologic examination up to 60 days posttreatment revealed scarring and spleen tissue regeneration at the treatment site. Conclusion: HIFU provides an effective and safe method of achieving hemostasis after acute splenic injury.

Original languageEnglish (US)
Pages (from-to)1115-1120
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Issue number6
StatePublished - Dec 1 2002
Externally publishedYes


  • Acoustic hemostasis
  • High-intensity focused ultrasound (HIFU)
  • Safety
  • Splenic trauma
  • Survival
  • Therapeutic ultrasound

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine


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