Abstract
Histamine elicits the sensation of itch at the site of skin application as well as alloknesis (itch elicited by innocuous mechanical stimuli) in a surrounding area in humans and expansion of the low-threshold mechanosensitive receptive field area of spinal wide dynamic range (WDR)- type dorsal horn neurons in rats. We presently tested if the histamine- evoked expansion of neuronal receptive field area depends on a spinal N- methyl-D-aspartate (NMDA) receptor-mediated process. In pentobarbital sodium- anesthetized rats, mechanical receptive field areas of single WDR-type dorsal horn neurons were mapped with graded von Frey filaments before and 10 min after intracutaneous (ic) microinjection of histamine (1 μl; 1, 3, or 10%) at a low-threshold site within the receptive field. Intracutaneous microinjection of histamine evoked dose-related increases in firing rate, as well as a dose-dependent expansion in mean receptive field area 10 min after 3 and 10%, but not 1%, histamine doses. When a noncompetitive or competitive NMDA receptor antagonist dizocilpine [MK-801; D(-)-2-amino-5- phosphonovalerate (APV), respectively; 1 μM] was first applied topically to the surface of the spinal cord, there was no significant change in mean receptive field area after ic microinjection of 10% histamine. The mean neuronal response to histamine in the presence of spinal MK-801 or APV was not significantly different from the mean response to histamine in the absence of these drugs. These results suggest that spinal NMDA receptors are involved in histamine-induced expansion of mechanical receptive field area, a neural event possibly involved in the development of alloknesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1613-1618 |
| Number of pages | 6 |
| Journal | Journal of Neurophysiology |
| Volume | 79 |
| Issue number | 4 |
| State | Published - 1998 |
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ASJC Scopus subject areas
- Physiology
- Neuroscience(all)
Cite this
Spinal NMDA receptor involvement in expansion of dorsal horn neuronal receptive field area produced by intracutaneous histamine. / Jinks, S. L.; Carstens, Earl.
In: Journal of Neurophysiology, Vol. 79, No. 4, 1998, p. 1613-1618.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spinal NMDA receptor involvement in expansion of dorsal horn neuronal receptive field area produced by intracutaneous histamine
AU - Jinks, S. L.
AU - Carstens, Earl
PY - 1998
Y1 - 1998
N2 - Histamine elicits the sensation of itch at the site of skin application as well as alloknesis (itch elicited by innocuous mechanical stimuli) in a surrounding area in humans and expansion of the low-threshold mechanosensitive receptive field area of spinal wide dynamic range (WDR)- type dorsal horn neurons in rats. We presently tested if the histamine- evoked expansion of neuronal receptive field area depends on a spinal N- methyl-D-aspartate (NMDA) receptor-mediated process. In pentobarbital sodium- anesthetized rats, mechanical receptive field areas of single WDR-type dorsal horn neurons were mapped with graded von Frey filaments before and 10 min after intracutaneous (ic) microinjection of histamine (1 μl; 1, 3, or 10%) at a low-threshold site within the receptive field. Intracutaneous microinjection of histamine evoked dose-related increases in firing rate, as well as a dose-dependent expansion in mean receptive field area 10 min after 3 and 10%, but not 1%, histamine doses. When a noncompetitive or competitive NMDA receptor antagonist dizocilpine [MK-801; D(-)-2-amino-5- phosphonovalerate (APV), respectively; 1 μM] was first applied topically to the surface of the spinal cord, there was no significant change in mean receptive field area after ic microinjection of 10% histamine. The mean neuronal response to histamine in the presence of spinal MK-801 or APV was not significantly different from the mean response to histamine in the absence of these drugs. These results suggest that spinal NMDA receptors are involved in histamine-induced expansion of mechanical receptive field area, a neural event possibly involved in the development of alloknesis.
AB - Histamine elicits the sensation of itch at the site of skin application as well as alloknesis (itch elicited by innocuous mechanical stimuli) in a surrounding area in humans and expansion of the low-threshold mechanosensitive receptive field area of spinal wide dynamic range (WDR)- type dorsal horn neurons in rats. We presently tested if the histamine- evoked expansion of neuronal receptive field area depends on a spinal N- methyl-D-aspartate (NMDA) receptor-mediated process. In pentobarbital sodium- anesthetized rats, mechanical receptive field areas of single WDR-type dorsal horn neurons were mapped with graded von Frey filaments before and 10 min after intracutaneous (ic) microinjection of histamine (1 μl; 1, 3, or 10%) at a low-threshold site within the receptive field. Intracutaneous microinjection of histamine evoked dose-related increases in firing rate, as well as a dose-dependent expansion in mean receptive field area 10 min after 3 and 10%, but not 1%, histamine doses. When a noncompetitive or competitive NMDA receptor antagonist dizocilpine [MK-801; D(-)-2-amino-5- phosphonovalerate (APV), respectively; 1 μM] was first applied topically to the surface of the spinal cord, there was no significant change in mean receptive field area after ic microinjection of 10% histamine. The mean neuronal response to histamine in the presence of spinal MK-801 or APV was not significantly different from the mean response to histamine in the absence of these drugs. These results suggest that spinal NMDA receptors are involved in histamine-induced expansion of mechanical receptive field area, a neural event possibly involved in the development of alloknesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031944069&partnerID=8YFLogxK
M3 - Article
C2 - 9535932
AN - SCOPUS:0031944069
VL - 79
SP - 1613
EP - 1618
JO - Journal of Neurophysiology
JF - Journal of Neurophysiology
SN - 0022-3077
IS - 4
ER -