Spinal cord involvement in the nonhuman primate model of Lyme disease

Yunhong Bai, Kavitha Narayan, Donna Dail, Marie Sondey, Emir Hodzic, Stephen W Barthold, Andrew R. Pachner, Diego Cadavid

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Lyme borreliosis is a multisystemic disease caused by infection with various genospecies of the spirochete Borrelia burgdorferi. The organs most often affected are the skin, joints, the heart, and the central and peripheral nervous systems. Multiple neurological complications can occur, including aseptic meningitis, encephalopathy, facial nerve palsy, radiculitis, myelitis, and peripheral neuropathy. To investigate spinal cord involvement in the nonhuman primate (NHP) model of Lyme borreliosis, we inoculated 25 adult Macaca mulatta with B. burgdorferi sensu strictu strains N40 by needle (N = 9) or by tick (N = 4) or 297 by needle (N = 2), or with B. burgdorferi genospecies garinii strains Pbi (N = 4), 793 (N = 2), or Pli (N = 4) by needle, Immunosuppression either transiently (TISP) or permanently (IS) was used to facilitate establishment of infection. Tissues and fluids were collected at necropsy 7-24 weeks later. Hematoxylin and eosin staining was used to study inflammation, and immunohistochemistry and digital image analysis to measure inflammation and localize spirochetes. The spirochetal load and C1q expression were measured by TaqMan RT-PCR. The results showed meningoradiculitis developed in only one of the 25 NHP's examined, TISP NHP 321 inoculated with B. garinii strain Pbi. Inflammation was localized to nerve roots, dorsal root ganglia, and leptomeninges but rarely to the spinal cord parenchyma itself. T cells and plasma cells were the predominant inflammatory cells. Significantly increased amounts of IgG, IgM, and C1q were found in inflamed spinal cord. Taqman RT-PCR found spirochetes in the spinal cord only in IS-NHP's, mostly in nerve roots and ganglia rather than in the cord parenchyma. C1q mRNA expression was significantly increased in inflamed spinal cord. This is the first comprehensive study of spinal cord involvement in Lyme borreliosis.

Original languageEnglish (US)
Pages (from-to)160-172
Number of pages13
JournalLaboratory Investigation
Volume84
Issue number2
DOIs
StatePublished - 2004

Fingerprint

Lyme Disease
Primates
Spinal Cord
Spirochaetales
Borrelia burgdorferi
Needles
Inflammation
Myelitis
Aseptic Meningitis
Polymerase Chain Reaction
Radiculopathy
Facial Paralysis
Facial Nerve
Peripheral Nervous System
Spinal Ganglia
Brain Diseases
Peripheral Nervous System Diseases
Ticks
Hematoxylin
Eosine Yellowish-(YS)

Keywords

  • Antibody
  • Borrelia burgdorferi
  • c1q
  • Garini
  • Lyme borreliosis
  • Spinal cord
  • Strains

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Spinal cord involvement in the nonhuman primate model of Lyme disease. / Bai, Yunhong; Narayan, Kavitha; Dail, Donna; Sondey, Marie; Hodzic, Emir; Barthold, Stephen W; Pachner, Andrew R.; Cadavid, Diego.

In: Laboratory Investigation, Vol. 84, No. 2, 2004, p. 160-172.

Research output: Contribution to journalArticle

Bai, Y, Narayan, K, Dail, D, Sondey, M, Hodzic, E, Barthold, SW, Pachner, AR & Cadavid, D 2004, 'Spinal cord involvement in the nonhuman primate model of Lyme disease', Laboratory Investigation, vol. 84, no. 2, pp. 160-172. https://doi.org/10.1038/labinvest.3700024
Bai, Yunhong ; Narayan, Kavitha ; Dail, Donna ; Sondey, Marie ; Hodzic, Emir ; Barthold, Stephen W ; Pachner, Andrew R. ; Cadavid, Diego. / Spinal cord involvement in the nonhuman primate model of Lyme disease. In: Laboratory Investigation. 2004 ; Vol. 84, No. 2. pp. 160-172.
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