Abstract
Lyme borreliosis is a multisystemic disease caused by infection with various genospecies of the spirochete Borrelia burgdorferi. The organs most often affected are the skin, joints, the heart, and the central and peripheral nervous systems. Multiple neurological complications can occur, including aseptic meningitis, encephalopathy, facial nerve palsy, radiculitis, myelitis, and peripheral neuropathy. To investigate spinal cord involvement in the nonhuman primate (NHP) model of Lyme borreliosis, we inoculated 25 adult Macaca mulatta with B. burgdorferi sensu strictu strains N40 by needle (N = 9) or by tick (N = 4) or 297 by needle (N = 2), or with B. burgdorferi genospecies garinii strains Pbi (N = 4), 793 (N = 2), or Pli (N = 4) by needle, Immunosuppression either transiently (TISP) or permanently (IS) was used to facilitate establishment of infection. Tissues and fluids were collected at necropsy 7-24 weeks later. Hematoxylin and eosin staining was used to study inflammation, and immunohistochemistry and digital image analysis to measure inflammation and localize spirochetes. The spirochetal load and C1q expression were measured by TaqMan RT-PCR. The results showed meningoradiculitis developed in only one of the 25 NHP's examined, TISP NHP 321 inoculated with B. garinii strain Pbi. Inflammation was localized to nerve roots, dorsal root ganglia, and leptomeninges but rarely to the spinal cord parenchyma itself. T cells and plasma cells were the predominant inflammatory cells. Significantly increased amounts of IgG, IgM, and C1q were found in inflamed spinal cord. Taqman RT-PCR found spirochetes in the spinal cord only in IS-NHP's, mostly in nerve roots and ganglia rather than in the cord parenchyma. C1q mRNA expression was significantly increased in inflamed spinal cord. This is the first comprehensive study of spinal cord involvement in Lyme borreliosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 160-172 |
| Number of pages | 13 |
| Journal | Laboratory Investigation |
| Volume | 84 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2004 |
Fingerprint
Keywords
- Antibody
- Borrelia burgdorferi
- c1q
- Garini
- Lyme borreliosis
- Spinal cord
- Strains
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Spinal cord involvement in the nonhuman primate model of Lyme disease. / Bai, Yunhong; Narayan, Kavitha; Dail, Donna; Sondey, Marie; Hodzic, Emir; Barthold, Stephen W; Pachner, Andrew R.; Cadavid, Diego.
In: Laboratory Investigation, Vol. 84, No. 2, 2004, p. 160-172.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spinal cord involvement in the nonhuman primate model of Lyme disease
AU - Bai, Yunhong
AU - Narayan, Kavitha
AU - Dail, Donna
AU - Sondey, Marie
AU - Hodzic, Emir
AU - Barthold, Stephen W
AU - Pachner, Andrew R.
AU - Cadavid, Diego
PY - 2004
Y1 - 2004
N2 - Lyme borreliosis is a multisystemic disease caused by infection with various genospecies of the spirochete Borrelia burgdorferi. The organs most often affected are the skin, joints, the heart, and the central and peripheral nervous systems. Multiple neurological complications can occur, including aseptic meningitis, encephalopathy, facial nerve palsy, radiculitis, myelitis, and peripheral neuropathy. To investigate spinal cord involvement in the nonhuman primate (NHP) model of Lyme borreliosis, we inoculated 25 adult Macaca mulatta with B. burgdorferi sensu strictu strains N40 by needle (N = 9) or by tick (N = 4) or 297 by needle (N = 2), or with B. burgdorferi genospecies garinii strains Pbi (N = 4), 793 (N = 2), or Pli (N = 4) by needle, Immunosuppression either transiently (TISP) or permanently (IS) was used to facilitate establishment of infection. Tissues and fluids were collected at necropsy 7-24 weeks later. Hematoxylin and eosin staining was used to study inflammation, and immunohistochemistry and digital image analysis to measure inflammation and localize spirochetes. The spirochetal load and C1q expression were measured by TaqMan RT-PCR. The results showed meningoradiculitis developed in only one of the 25 NHP's examined, TISP NHP 321 inoculated with B. garinii strain Pbi. Inflammation was localized to nerve roots, dorsal root ganglia, and leptomeninges but rarely to the spinal cord parenchyma itself. T cells and plasma cells were the predominant inflammatory cells. Significantly increased amounts of IgG, IgM, and C1q were found in inflamed spinal cord. Taqman RT-PCR found spirochetes in the spinal cord only in IS-NHP's, mostly in nerve roots and ganglia rather than in the cord parenchyma. C1q mRNA expression was significantly increased in inflamed spinal cord. This is the first comprehensive study of spinal cord involvement in Lyme borreliosis.
AB - Lyme borreliosis is a multisystemic disease caused by infection with various genospecies of the spirochete Borrelia burgdorferi. The organs most often affected are the skin, joints, the heart, and the central and peripheral nervous systems. Multiple neurological complications can occur, including aseptic meningitis, encephalopathy, facial nerve palsy, radiculitis, myelitis, and peripheral neuropathy. To investigate spinal cord involvement in the nonhuman primate (NHP) model of Lyme borreliosis, we inoculated 25 adult Macaca mulatta with B. burgdorferi sensu strictu strains N40 by needle (N = 9) or by tick (N = 4) or 297 by needle (N = 2), or with B. burgdorferi genospecies garinii strains Pbi (N = 4), 793 (N = 2), or Pli (N = 4) by needle, Immunosuppression either transiently (TISP) or permanently (IS) was used to facilitate establishment of infection. Tissues and fluids were collected at necropsy 7-24 weeks later. Hematoxylin and eosin staining was used to study inflammation, and immunohistochemistry and digital image analysis to measure inflammation and localize spirochetes. The spirochetal load and C1q expression were measured by TaqMan RT-PCR. The results showed meningoradiculitis developed in only one of the 25 NHP's examined, TISP NHP 321 inoculated with B. garinii strain Pbi. Inflammation was localized to nerve roots, dorsal root ganglia, and leptomeninges but rarely to the spinal cord parenchyma itself. T cells and plasma cells were the predominant inflammatory cells. Significantly increased amounts of IgG, IgM, and C1q were found in inflamed spinal cord. Taqman RT-PCR found spirochetes in the spinal cord only in IS-NHP's, mostly in nerve roots and ganglia rather than in the cord parenchyma. C1q mRNA expression was significantly increased in inflamed spinal cord. This is the first comprehensive study of spinal cord involvement in Lyme borreliosis.
KW - Antibody
KW - Borrelia burgdorferi
KW - c1q
KW - Garini
KW - Lyme borreliosis
KW - Spinal cord
KW - Strains
UR - http://www.scopus.com/inward/record.url?scp=1542504571&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1542504571&partnerID=8YFLogxK
U2 - 10.1038/labinvest.3700024
DO - 10.1038/labinvest.3700024
M3 - Article
C2 - 14688796
AN - SCOPUS:1542504571
VL - 84
SP - 160
EP - 172
JO - Laboratory Investigation
JF - Laboratory Investigation
SN - 0023-6837
IS - 2
ER -