Sphingosine-1-phosphate Maintains Normal Vascular Permeability by Preserving Endothelial Surface Glycocalyx in Intact Microvessels

Lin Zhang, Min Zeng, Jie Fan, John M. Tarbell, Fitz Roy E Curry, Bingmei M. Fu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective: S1P was found to protect the ESG by inhibiting MMP activity-dependent shedding of ESG in cultured endothelial cell studies. We aimed to further test that S1P contributes to the maintenance of normal vascular permeability by protecting the ESG in intact microvessels. Methods: We quantified the ESG in post-capillary venules of rat mesentery and measured the vascular permeability to albumin in the presence and absence of 1 μM S1P. We also measured permeability to albumin in the presence of MMP inhibitors and compared the measured permeability with those predicted by a transport model for the inter-endothelial cleft. Results: We found that in the absence of S1P, the fluorescence intensity of the FITC-anti–HS-labeled ESG was ~10% of that in the presence of S1P, whereas the measured permeability to albumin was ~6.5-fold of that in the presence of S1P. Similar results were observed with MMP inhibition. The predictions by the mathematical model further confirmed that S1P maintains microvascular permeability by preserving ESG. Conclusions: Our results show that S1P contributes to the maintenance of normal vascular permeability by protecting the ESG in intact microvessels, consistent with parallel observation in cultured endothelial monolayers.

Original languageEnglish (US)
Pages (from-to)301-310
Number of pages10
JournalMicrocirculation
Volume23
Issue number4
DOIs
StatePublished - 2016

Fingerprint

Glycocalyx
Capillary Permeability
Microvessels
Albumins
Permeability
Matrix Metalloproteinases
Maintenance
Mesentery
Matrix Metalloproteinase Inhibitors
Venules
Fluorescein-5-isothiocyanate
Cultured Cells
Theoretical Models
Endothelial Cells
Fluorescence
Observation
sphingosine 1-phosphate

Keywords

  • heparan sulfate
  • in situ immunostaining
  • matrix metalloproteinase
  • rat mesentery
  • transport model for the inter-endothelial cleft

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Sphingosine-1-phosphate Maintains Normal Vascular Permeability by Preserving Endothelial Surface Glycocalyx in Intact Microvessels. / Zhang, Lin; Zeng, Min; Fan, Jie; Tarbell, John M.; Curry, Fitz Roy E; Fu, Bingmei M.

In: Microcirculation, Vol. 23, No. 4, 2016, p. 301-310.

Research output: Contribution to journalArticle

Zhang, Lin ; Zeng, Min ; Fan, Jie ; Tarbell, John M. ; Curry, Fitz Roy E ; Fu, Bingmei M. / Sphingosine-1-phosphate Maintains Normal Vascular Permeability by Preserving Endothelial Surface Glycocalyx in Intact Microvessels. In: Microcirculation. 2016 ; Vol. 23, No. 4. pp. 301-310.
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