Abstract
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1920-1931 |
| Number of pages | 12 |
| Journal | Journal of Lipid Research |
| Volume | 53 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2012 |
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Keywords
- Colon cancer
- Development
- Signal transduction
- Sphingolipid
- Sphingosine phosphate lyase
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Endocrinology
Cite this
Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues. / Borowsky, Alexander D; Bandhuvula, Padmavathi; Kumar, Ashok; Yoshinaga, Yuko; Nefedov, Mikhail; Fong, Loren G.; Zhang, Meng; Baridon, Brian; Dillard, Lisa; De Jong, Pieter; Young, Stephen G.; West, David B.; Saba, Julie D.
In: Journal of Lipid Research, Vol. 53, No. 9, 09.2012, p. 1920-1931.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues
AU - Borowsky, Alexander D
AU - Bandhuvula, Padmavathi
AU - Kumar, Ashok
AU - Yoshinaga, Yuko
AU - Nefedov, Mikhail
AU - Fong, Loren G.
AU - Zhang, Meng
AU - Baridon, Brian
AU - Dillard, Lisa
AU - De Jong, Pieter
AU - Young, Stephen G.
AU - West, David B.
AU - Saba, Julie D.
PY - 2012/9
Y1 - 2012/9
N2 - Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.
AB - Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.
KW - Colon cancer
KW - Development
KW - Signal transduction
KW - Sphingolipid
KW - Sphingosine phosphate lyase
UR - http://www.scopus.com/inward/record.url?scp=84864863230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864863230&partnerID=8YFLogxK
U2 - 10.1194/jlr.M028084
DO - 10.1194/jlr.M028084
M3 - Article
C2 - 22781001
AN - SCOPUS:84864863230
VL - 53
SP - 1920
EP - 1931
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 9
ER -