Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues

Alexander D Borowsky, Padmavathi Bandhuvula, Ashok Kumar, Yuko Yoshinaga, Mikhail Nefedov, Loren G. Fong, Meng Zhang, Brian Baridon, Lisa Dillard, Pieter De Jong, Stephen G. Young, David B. West, Julie D. Saba

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

Original languageEnglish (US)
Pages (from-to)1920-1931
Number of pages12
JournalJournal of Lipid Research
Volume53
Issue number9
DOIs
StatePublished - Sep 2012

Fingerprint

Lyases
Tissue
Lymphocytes
Immunity
Harderian Gland
Galactosidases
Arachnoid
sphingosine 1-phosphate lyase (aldolase)
Peyer's Patches
Trigeminal Ganglion
Rhombencephalon
Sphingolipids
Trigeminal Nerve
Choroid Plexus
Brown Adipose Tissue
Hair Follicle
Olfactory Bulb
Adrenal Cortex
Gonads
Enzyme Assays

Keywords

  • Colon cancer
  • Development
  • Signal transduction
  • Sphingolipid
  • Sphingosine phosphate lyase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Borowsky, A. D., Bandhuvula, P., Kumar, A., Yoshinaga, Y., Nefedov, M., Fong, L. G., ... Saba, J. D. (2012). Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues. Journal of Lipid Research, 53(9), 1920-1931. https://doi.org/10.1194/jlr.M028084

Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues. / Borowsky, Alexander D; Bandhuvula, Padmavathi; Kumar, Ashok; Yoshinaga, Yuko; Nefedov, Mikhail; Fong, Loren G.; Zhang, Meng; Baridon, Brian; Dillard, Lisa; De Jong, Pieter; Young, Stephen G.; West, David B.; Saba, Julie D.

In: Journal of Lipid Research, Vol. 53, No. 9, 09.2012, p. 1920-1931.

Research output: Contribution to journalArticle

Borowsky, AD, Bandhuvula, P, Kumar, A, Yoshinaga, Y, Nefedov, M, Fong, LG, Zhang, M, Baridon, B, Dillard, L, De Jong, P, Young, SG, West, DB & Saba, JD 2012, 'Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues', Journal of Lipid Research, vol. 53, no. 9, pp. 1920-1931. https://doi.org/10.1194/jlr.M028084
Borowsky, Alexander D ; Bandhuvula, Padmavathi ; Kumar, Ashok ; Yoshinaga, Yuko ; Nefedov, Mikhail ; Fong, Loren G. ; Zhang, Meng ; Baridon, Brian ; Dillard, Lisa ; De Jong, Pieter ; Young, Stephen G. ; West, David B. ; Saba, Julie D. / Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues. In: Journal of Lipid Research. 2012 ; Vol. 53, No. 9. pp. 1920-1931.
@article{54c28cd80fd5447dabb64eebe609d4be,
title = "Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues",
abstract = "Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.",
keywords = "Colon cancer, Development, Signal transduction, Sphingolipid, Sphingosine phosphate lyase",
author = "Borowsky, {Alexander D} and Padmavathi Bandhuvula and Ashok Kumar and Yuko Yoshinaga and Mikhail Nefedov and Fong, {Loren G.} and Meng Zhang and Brian Baridon and Lisa Dillard and {De Jong}, Pieter and Young, {Stephen G.} and West, {David B.} and Saba, {Julie D.}",
year = "2012",
month = "9",
doi = "10.1194/jlr.M028084",
language = "English (US)",
volume = "53",
pages = "1920--1931",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues

AU - Borowsky, Alexander D

AU - Bandhuvula, Padmavathi

AU - Kumar, Ashok

AU - Yoshinaga, Yuko

AU - Nefedov, Mikhail

AU - Fong, Loren G.

AU - Zhang, Meng

AU - Baridon, Brian

AU - Dillard, Lisa

AU - De Jong, Pieter

AU - Young, Stephen G.

AU - West, David B.

AU - Saba, Julie D.

PY - 2012/9

Y1 - 2012/9

N2 - Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

AB - Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

KW - Colon cancer

KW - Development

KW - Signal transduction

KW - Sphingolipid

KW - Sphingosine phosphate lyase

UR - http://www.scopus.com/inward/record.url?scp=84864863230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864863230&partnerID=8YFLogxK

U2 - 10.1194/jlr.M028084

DO - 10.1194/jlr.M028084

M3 - Article

C2 - 22781001

AN - SCOPUS:84864863230

VL - 53

SP - 1920

EP - 1931

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 9

ER -