Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues

Alexander D Borowsky, Padmavathi Bandhuvula, Ashok Kumar, Yuko Yoshinaga, Mikhail Nefedov, Loren G. Fong, Meng Zhang, Brian Baridon, Lisa Dillard, Pieter De Jong, Stephen G. Young, David B. West, Julie D. Saba

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in immunity, inflammation, angiogenesis, and cancer. S1P lyase (SPL) is the essential enzyme responsible for S1P degradation. SPL augments apoptosis and is down-regulated in cancer. SPL generates a S1P chemical gradient that promotes lymphocyte trafficking and as such is being targeted to treat autoimmune diseases. Despite growing interest in SPL as a disease marker, antioncogene, and pharmacological target, no comprehensive characterization of SPL expression in mammalian tissues has been reported. We investigated SPL expression in developing and adult mouse tissues by generating and characterizing a β-galactosidase-SPL reporter mouse combined with immunohistochemistry, immunoblotting, and enzyme assays. SPL was expressed in thymic and splenic stromal cells, splenocytes, Peyer's Patches, colonic lymphoid aggregates, circulating T and B lymphocytes, granulocytes, and monocytes, with lowest expression in thymocytes. SPL was highly expressed within the CNS, including arachnoid lining cells, spinal cord, choroid plexus, trigeminal nerve ganglion, and specific neurons of the olfactory bulb, cerebral cortex, midbrain, hindbrain, and cerebellum. Expression was detected in brown adipose tissue, female gonads, adrenal cortex, bladder epithelium, Harderian and preputial glands, and hair follicles. This unique expression pattern suggests SPL has many undiscovered physiological functions apart from its role in immunity.

Original languageEnglish (US)
Pages (from-to)1920-1931
Number of pages12
JournalJournal of Lipid Research
Volume53
Issue number9
DOIs
StatePublished - Sep 2012

Keywords

  • Colon cancer
  • Development
  • Signal transduction
  • Sphingolipid
  • Sphingosine phosphate lyase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues'. Together they form a unique fingerprint.

  • Cite this

    Borowsky, A. D., Bandhuvula, P., Kumar, A., Yoshinaga, Y., Nefedov, M., Fong, L. G., Zhang, M., Baridon, B., Dillard, L., De Jong, P., Young, S. G., West, D. B., & Saba, J. D. (2012). Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues. Journal of Lipid Research, 53(9), 1920-1931. https://doi.org/10.1194/jlr.M028084