Sperm-zona pellucida interaction in cynomolgus and rhesus macaques

C. A. Vandevoort, Theodore L Tollner, J. W. Overstreet

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

These experiments were carried out to establish and validate an in vitro system for studying macaque sperm-zona pellucida interaction. Sperm of rhesus and cynomolgus macaques were capacitated in vitro and incubated with cryopreserved zonae pellucidae. Homologous gamete incubations were tested, as well as cross-species combinations. Approximately 25% of macaque sperm bound to the zonae acrosome reacted within 1 minute of gamete coincubation, although the percentage of acrosome reactions in the sperm suspension was less than 1%. There was a small but consistent increase in the percent of acrosome reactions of zona sperm after an additional hour of incubation in sperm-free media. Similar results were obtained in the cross-species experiments, suggesting that zonae from the two macaque species can be used interchangeably in sperm-zona binding assays. Differences in the physiologic characteristics of the sperm of the macaque species were demonstrated. Cynomolgus sperm required activation with caffeine and dibutyryl cyclic adenosine monophosphate (dbcAMP) in order to bind to the zonae. Rhesus sperm were able to bind to the zonae and acrosome react in the absence of activators, although both sperm binding and percentage of acrosome reactions increased with the addition of activators. Large numbers of sperm from both macaque species bound to the zonae of hamster oocytes after treatment with activators, but the bound sperm did not acrosome react. These experiments demonstrate the importance of evaluating the acrosomal status of sperm when sperm-zona binding assays are performed with macaque gametes.

Original languageEnglish (US)
Pages (from-to)428-432
Number of pages5
JournalJournal of Andrology
Volume13
Issue number5
StatePublished - 1992

Fingerprint

Zona Pellucida
Macaca mulatta
Spermatozoa
Herpes Zoster
Macaca
Acrosome Reaction
Acrosome
Germ Cells
Sperm Count
Caffeine
Cricetinae
Cyclic AMP
Oocytes

Keywords

  • acrosome reaction
  • caffeine
  • dbcAMP
  • semen

ASJC Scopus subject areas

  • Endocrinology

Cite this

Sperm-zona pellucida interaction in cynomolgus and rhesus macaques. / Vandevoort, C. A.; Tollner, Theodore L; Overstreet, J. W.

In: Journal of Andrology, Vol. 13, No. 5, 1992, p. 428-432.

Research output: Contribution to journalArticle

Vandevoort, CA, Tollner, TL & Overstreet, JW 1992, 'Sperm-zona pellucida interaction in cynomolgus and rhesus macaques', Journal of Andrology, vol. 13, no. 5, pp. 428-432.
Vandevoort, C. A. ; Tollner, Theodore L ; Overstreet, J. W. / Sperm-zona pellucida interaction in cynomolgus and rhesus macaques. In: Journal of Andrology. 1992 ; Vol. 13, No. 5. pp. 428-432.
@article{59f9ba937de2442c8507c08dce8bc737,
title = "Sperm-zona pellucida interaction in cynomolgus and rhesus macaques",
abstract = "These experiments were carried out to establish and validate an in vitro system for studying macaque sperm-zona pellucida interaction. Sperm of rhesus and cynomolgus macaques were capacitated in vitro and incubated with cryopreserved zonae pellucidae. Homologous gamete incubations were tested, as well as cross-species combinations. Approximately 25{\%} of macaque sperm bound to the zonae acrosome reacted within 1 minute of gamete coincubation, although the percentage of acrosome reactions in the sperm suspension was less than 1{\%}. There was a small but consistent increase in the percent of acrosome reactions of zona sperm after an additional hour of incubation in sperm-free media. Similar results were obtained in the cross-species experiments, suggesting that zonae from the two macaque species can be used interchangeably in sperm-zona binding assays. Differences in the physiologic characteristics of the sperm of the macaque species were demonstrated. Cynomolgus sperm required activation with caffeine and dibutyryl cyclic adenosine monophosphate (dbcAMP) in order to bind to the zonae. Rhesus sperm were able to bind to the zonae and acrosome react in the absence of activators, although both sperm binding and percentage of acrosome reactions increased with the addition of activators. Large numbers of sperm from both macaque species bound to the zonae of hamster oocytes after treatment with activators, but the bound sperm did not acrosome react. These experiments demonstrate the importance of evaluating the acrosomal status of sperm when sperm-zona binding assays are performed with macaque gametes.",
keywords = "acrosome reaction, caffeine, dbcAMP, semen",
author = "Vandevoort, {C. A.} and Tollner, {Theodore L} and Overstreet, {J. W.}",
year = "1992",
language = "English (US)",
volume = "13",
pages = "428--432",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "5",

}

TY - JOUR

T1 - Sperm-zona pellucida interaction in cynomolgus and rhesus macaques

AU - Vandevoort, C. A.

AU - Tollner, Theodore L

AU - Overstreet, J. W.

PY - 1992

Y1 - 1992

N2 - These experiments were carried out to establish and validate an in vitro system for studying macaque sperm-zona pellucida interaction. Sperm of rhesus and cynomolgus macaques were capacitated in vitro and incubated with cryopreserved zonae pellucidae. Homologous gamete incubations were tested, as well as cross-species combinations. Approximately 25% of macaque sperm bound to the zonae acrosome reacted within 1 minute of gamete coincubation, although the percentage of acrosome reactions in the sperm suspension was less than 1%. There was a small but consistent increase in the percent of acrosome reactions of zona sperm after an additional hour of incubation in sperm-free media. Similar results were obtained in the cross-species experiments, suggesting that zonae from the two macaque species can be used interchangeably in sperm-zona binding assays. Differences in the physiologic characteristics of the sperm of the macaque species were demonstrated. Cynomolgus sperm required activation with caffeine and dibutyryl cyclic adenosine monophosphate (dbcAMP) in order to bind to the zonae. Rhesus sperm were able to bind to the zonae and acrosome react in the absence of activators, although both sperm binding and percentage of acrosome reactions increased with the addition of activators. Large numbers of sperm from both macaque species bound to the zonae of hamster oocytes after treatment with activators, but the bound sperm did not acrosome react. These experiments demonstrate the importance of evaluating the acrosomal status of sperm when sperm-zona binding assays are performed with macaque gametes.

AB - These experiments were carried out to establish and validate an in vitro system for studying macaque sperm-zona pellucida interaction. Sperm of rhesus and cynomolgus macaques were capacitated in vitro and incubated with cryopreserved zonae pellucidae. Homologous gamete incubations were tested, as well as cross-species combinations. Approximately 25% of macaque sperm bound to the zonae acrosome reacted within 1 minute of gamete coincubation, although the percentage of acrosome reactions in the sperm suspension was less than 1%. There was a small but consistent increase in the percent of acrosome reactions of zona sperm after an additional hour of incubation in sperm-free media. Similar results were obtained in the cross-species experiments, suggesting that zonae from the two macaque species can be used interchangeably in sperm-zona binding assays. Differences in the physiologic characteristics of the sperm of the macaque species were demonstrated. Cynomolgus sperm required activation with caffeine and dibutyryl cyclic adenosine monophosphate (dbcAMP) in order to bind to the zonae. Rhesus sperm were able to bind to the zonae and acrosome react in the absence of activators, although both sperm binding and percentage of acrosome reactions increased with the addition of activators. Large numbers of sperm from both macaque species bound to the zonae of hamster oocytes after treatment with activators, but the bound sperm did not acrosome react. These experiments demonstrate the importance of evaluating the acrosomal status of sperm when sperm-zona binding assays are performed with macaque gametes.

KW - acrosome reaction

KW - caffeine

KW - dbcAMP

KW - semen

UR - http://www.scopus.com/inward/record.url?scp=0026726813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026726813&partnerID=8YFLogxK

M3 - Article

C2 - 1331011

AN - SCOPUS:0026726813

VL - 13

SP - 428

EP - 432

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 5

ER -