Abstract
Whether the main energy source for sperm motility is from oxidative phosphorylation or glycolysis has been long-debated in the field of reproductive biology. Using the rhesus monkey as a model, we examined the role of glycolysis and oxidative phosphorylation in sperm function by using alpha-chlorohydrin (ACH), a glycolysis inhibitor, and pentachlorophenol (PCP), an oxidative phosphorylation uncoupler. Sperm treated with ACH showed no change in percentage of motile sperm, although sperm motion was impaired. The ACH-treated sperm did not display either hyperactivity- or hyperactivation-associated changes in protein tyrosine phosphorylation. When treated with PCP, sperm motion parameters were affected by the highest level of PCP (200 μM); however, PCP did not cause motility impairments even after chemical activation. Sperm treated with PCP were able to display hyperactivity and tyrosine phosphorylation after chemical activation. In contrast with motility measurements, treatment with either the glycolytic inhibitor or the oxidative phosphorylation inhibitor did not affect spermzona binding and zona-induced acrosome reaction. The results suggest glycolysis is essential to support sperm motility, hyperactivity, and protein tyrosine phosphorylation, while energy from oxidative phosphorylation is not necessary for hyperactivated sperm motility, tyrosine phosphorylation, spermzona binding, and acrosome reaction in the rhesus macaque.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 367-375 |
| Number of pages | 9 |
| Journal | Biology of Reproduction |
| Volume | 79 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 2008 |
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Keywords
- Acrosome reaction
- Sperm
- Sperm capacitation
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Embryology
Cite this
Sperm mitochondrial integrity is not required for hyperactivated motility, zona binding, or acrosome reaction in the rhesus macaque. / Hung, Pei Hsuan; Miller, Marion G.; Meyers, Stuart A; VandeVoort, Catherine A.
In: Biology of Reproduction, Vol. 79, No. 2, 08.2008, p. 367-375.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sperm mitochondrial integrity is not required for hyperactivated motility, zona binding, or acrosome reaction in the rhesus macaque
AU - Hung, Pei Hsuan
AU - Miller, Marion G.
AU - Meyers, Stuart A
AU - VandeVoort, Catherine A.
PY - 2008/8
Y1 - 2008/8
N2 - Whether the main energy source for sperm motility is from oxidative phosphorylation or glycolysis has been long-debated in the field of reproductive biology. Using the rhesus monkey as a model, we examined the role of glycolysis and oxidative phosphorylation in sperm function by using alpha-chlorohydrin (ACH), a glycolysis inhibitor, and pentachlorophenol (PCP), an oxidative phosphorylation uncoupler. Sperm treated with ACH showed no change in percentage of motile sperm, although sperm motion was impaired. The ACH-treated sperm did not display either hyperactivity- or hyperactivation-associated changes in protein tyrosine phosphorylation. When treated with PCP, sperm motion parameters were affected by the highest level of PCP (200 μM); however, PCP did not cause motility impairments even after chemical activation. Sperm treated with PCP were able to display hyperactivity and tyrosine phosphorylation after chemical activation. In contrast with motility measurements, treatment with either the glycolytic inhibitor or the oxidative phosphorylation inhibitor did not affect spermzona binding and zona-induced acrosome reaction. The results suggest glycolysis is essential to support sperm motility, hyperactivity, and protein tyrosine phosphorylation, while energy from oxidative phosphorylation is not necessary for hyperactivated sperm motility, tyrosine phosphorylation, spermzona binding, and acrosome reaction in the rhesus macaque.
AB - Whether the main energy source for sperm motility is from oxidative phosphorylation or glycolysis has been long-debated in the field of reproductive biology. Using the rhesus monkey as a model, we examined the role of glycolysis and oxidative phosphorylation in sperm function by using alpha-chlorohydrin (ACH), a glycolysis inhibitor, and pentachlorophenol (PCP), an oxidative phosphorylation uncoupler. Sperm treated with ACH showed no change in percentage of motile sperm, although sperm motion was impaired. The ACH-treated sperm did not display either hyperactivity- or hyperactivation-associated changes in protein tyrosine phosphorylation. When treated with PCP, sperm motion parameters were affected by the highest level of PCP (200 μM); however, PCP did not cause motility impairments even after chemical activation. Sperm treated with PCP were able to display hyperactivity and tyrosine phosphorylation after chemical activation. In contrast with motility measurements, treatment with either the glycolytic inhibitor or the oxidative phosphorylation inhibitor did not affect spermzona binding and zona-induced acrosome reaction. The results suggest glycolysis is essential to support sperm motility, hyperactivity, and protein tyrosine phosphorylation, while energy from oxidative phosphorylation is not necessary for hyperactivated sperm motility, tyrosine phosphorylation, spermzona binding, and acrosome reaction in the rhesus macaque.
KW - Acrosome reaction
KW - Sperm
KW - Sperm capacitation
UR - http://www.scopus.com/inward/record.url?scp=48749097605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48749097605&partnerID=8YFLogxK
U2 - 10.1095/biolreprod.107.066357
DO - 10.1095/biolreprod.107.066357
M3 - Article
C2 - 18480469
AN - SCOPUS:48749097605
VL - 79
SP - 367
EP - 375
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 2
ER -