Sperm Mitochondrial Function is Affected by Stallion Age and Predicts Post-Thaw Motility

Christa R. Darr, Luis E. Moraes, Tawny N. Scanlan, Julie Baumber-Skaife, Paul R. Loomis, Gino A Cortopassi, Stuart A Meyers

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Sperm motility and quality decline with stallion age and sperm preservation, but the mechanisms of functional deficit have not been explained. We tested the hypothesis that mitochondrial deficits underlie age- and cryopreservation-related deficits in stallion fertility by measuring mitochondrial function, motility, and reactive oxygen species (ROS) in 88 frozen-thawed ejaculates from 19 stallions of varying ages. As expected with increasing age, total sperm motility, progressive motility, and average path velocity decreased, and ROS production increased. For every unit increase in oxygen consumption, there was a 77% increase in the odds of sperm movement (P < .05), confirming the link between mitochondrial functionality and motility. In addition, the rate of mitochondrial oxygen consumption increased from 4 years of age to a peak at 12 years of age and decreased steadily thereafter (P < .05). This confirms the importance of mitochondrial functionality for overall sperm health and motility, implicating mitochondrial dysfunction as a major contributor to sperm aging and cryopreservation damage.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalJournal of Equine Veterinary Science
Volume50
DOIs
StatePublished - Mar 1 2017

Fingerprint

Sperm Motility
stallions
Spermatozoa
Cryopreservation
spermatozoa
Oxygen Consumption
Reactive Oxygen Species
Semen Preservation
sperm motility
cryopreservation
oxygen consumption
Fertility
reactive oxygen species
Health

Keywords

  • Aging
  • Cryopreservation
  • Mitochondria
  • Sperm
  • Stallion

ASJC Scopus subject areas

  • Equine

Cite this

Sperm Mitochondrial Function is Affected by Stallion Age and Predicts Post-Thaw Motility. / Darr, Christa R.; Moraes, Luis E.; Scanlan, Tawny N.; Baumber-Skaife, Julie; Loomis, Paul R.; Cortopassi, Gino A; Meyers, Stuart A.

In: Journal of Equine Veterinary Science, Vol. 50, 01.03.2017, p. 52-61.

Research output: Contribution to journalArticle

Darr, Christa R. ; Moraes, Luis E. ; Scanlan, Tawny N. ; Baumber-Skaife, Julie ; Loomis, Paul R. ; Cortopassi, Gino A ; Meyers, Stuart A. / Sperm Mitochondrial Function is Affected by Stallion Age and Predicts Post-Thaw Motility. In: Journal of Equine Veterinary Science. 2017 ; Vol. 50. pp. 52-61.
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