Spectroscopy-based quantitative fluorescence resonance energy transfer analysis.

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Abstract

The combination of green fluorescent protein mutants and fluorescence resonance energy transfer (FRET) forms a powerful tool for ion channel studies. A key to successful application of green fluorescent protein-based FRET is to reliably separate the FRET signal from various non-FRET fluorescence emissions that coexist in any experimental system. This chapter introduces a FRET quantification method that is based on fluorescence spectroscopic microscopy. Application of this "spectra FRET" method to both the confocal imaging of Xenopus oocytes and the epifluorescence imaging of culture cells is described. The fluorescence intensity ratio measurement, a complementary non-FRET method for identifying the channel subunit stoichiometry, is also discussed.

Original languageEnglish (US)
Pages (from-to)65-77
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume337
StatePublished - 2006

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Fluorescence Resonance Energy Transfer
Spectrum Analysis
Green Fluorescent Proteins
Energy Transfer
Xenopus
Ion Channels
Fluorescence Microscopy
Oocytes
Cell Culture Techniques
Fluorescence

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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AB - The combination of green fluorescent protein mutants and fluorescence resonance energy transfer (FRET) forms a powerful tool for ion channel studies. A key to successful application of green fluorescent protein-based FRET is to reliably separate the FRET signal from various non-FRET fluorescence emissions that coexist in any experimental system. This chapter introduces a FRET quantification method that is based on fluorescence spectroscopic microscopy. Application of this "spectra FRET" method to both the confocal imaging of Xenopus oocytes and the epifluorescence imaging of culture cells is described. The fluorescence intensity ratio measurement, a complementary non-FRET method for identifying the channel subunit stoichiometry, is also discussed.

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