Spectroscopic study of the effect of laser heating on collagen stability: Implications for tissue welding

Ward Small IV, Luiz B. Da Silva, Dennis L Matthews

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Collagen is believed to play a major role in laser tissue welding. Furthermore, the fundamental fusion mechanism(s) may include thermal denaturation of the collagen fibers. An in vitro investigation of the effect of laser heating (1.9 μm diode laser) on collagen conformation was performed. Fourier transform infrared (FTIR) spectra of native and heated porcine cornea were obtained. A low-frequency shift in the amide A band of collagen around 3320 cm -1 indicated that conformational changes associated with denaturation occurred. Samples heated using a temperature feedback controlled 1.9 μm diode laser showed a gradual decrease in the amide A peak frequency with increasing temperature, as did samples heated in a water bath. Complete denaturation was achieved at temperatures above 85°C for both heating protocols. Water loss induced by laser heating may have reduced the mobility of the collagen polypeptide chains, and contributed to the elevated denaturation temperature.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.R. Anderson, K.E. Bartels, L.S. Bass, C.G. Garrett
Pages276-281
Number of pages6
Volume3245
DOIs
StatePublished - 1997
Externally publishedYes
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII - San Jose, CA, United States
Duration: Jan 24 1998Jan 25 1998

Other

OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII
CountryUnited States
CitySan Jose, CA
Period1/24/981/25/98

Fingerprint

Laser heating
laser heating
collagens
welding
Collagen
Denaturation
biopolymer denaturation
Welding
Tissue
Amides
amides
Semiconductor lasers
semiconductor lasers
water loss
Temperature
cornea
temperature
Polypeptides
polypeptides
frequency shift

Keywords

  • Cornea
  • Dehydration
  • Denaturation
  • Fourier transform infrared spectroscopy
  • Temperature feedback

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Small IV, W., Da Silva, L. B., & Matthews, D. L. (1997). Spectroscopic study of the effect of laser heating on collagen stability: Implications for tissue welding. In R. R. Anderson, K. E. Bartels, L. S. Bass, & C. G. Garrett (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3245, pp. 276-281) https://doi.org/10.1117/12.312298

Spectroscopic study of the effect of laser heating on collagen stability : Implications for tissue welding. / Small IV, Ward; Da Silva, Luiz B.; Matthews, Dennis L.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / R.R. Anderson; K.E. Bartels; L.S. Bass; C.G. Garrett. Vol. 3245 1997. p. 276-281.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Small IV, W, Da Silva, LB & Matthews, DL 1997, Spectroscopic study of the effect of laser heating on collagen stability: Implications for tissue welding. in RR Anderson, KE Bartels, LS Bass & CG Garrett (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 3245, pp. 276-281, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VIII, San Jose, CA, United States, 1/24/98. https://doi.org/10.1117/12.312298
Small IV W, Da Silva LB, Matthews DL. Spectroscopic study of the effect of laser heating on collagen stability: Implications for tissue welding. In Anderson RR, Bartels KE, Bass LS, Garrett CG, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3245. 1997. p. 276-281 https://doi.org/10.1117/12.312298
Small IV, Ward ; Da Silva, Luiz B. ; Matthews, Dennis L. / Spectroscopic study of the effect of laser heating on collagen stability : Implications for tissue welding. Proceedings of SPIE - The International Society for Optical Engineering. editor / R.R. Anderson ; K.E. Bartels ; L.S. Bass ; C.G. Garrett. Vol. 3245 1997. pp. 276-281
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