TY - JOUR
T1 - Spectroscopic imaging for detection of ischemic injury in rat kidneys by use of changes in intrinsic optical properties
AU - Michalopoulou, Andromachi P.
AU - Fitzgerald, Jason T.
AU - Troppmann, Christoph
AU - Demos, Stavros G.
PY - 2005/4/10
Y1 - 2005/4/10
N2 - It is currently impossible to consistently predict kidney graft viability and function before and after transplantation. We explored optical spectroscopy to assess the degree of ischemic damage in kidney tissue. Tunable UV laser excitation was used to record autofluorescence images, at different spectral ranges, of injured and contralateral control rat kidneys to reveal the excitation conditions that offered optimal contrast. Autofluorescence and near-infrared cross-polarized light-scattering imaging were both used to monitor changes in intensity and spectral characteristics, as a function of exposure time to ischemic injury. These two modalities provided different temporal behaviors, arguably arising from two different mechanisms providing direct correlation of intrinsic optical signatures to ischemic injury time.
AB - It is currently impossible to consistently predict kidney graft viability and function before and after transplantation. We explored optical spectroscopy to assess the degree of ischemic damage in kidney tissue. Tunable UV laser excitation was used to record autofluorescence images, at different spectral ranges, of injured and contralateral control rat kidneys to reveal the excitation conditions that offered optimal contrast. Autofluorescence and near-infrared cross-polarized light-scattering imaging were both used to monitor changes in intensity and spectral characteristics, as a function of exposure time to ischemic injury. These two modalities provided different temporal behaviors, arguably arising from two different mechanisms providing direct correlation of intrinsic optical signatures to ischemic injury time.
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U2 - 10.1364/AO.44.002024
DO - 10.1364/AO.44.002024
M3 - Article
C2 - 15835351
AN - SCOPUS:17644382957
VL - 44
SP - 2024
EP - 2032
JO - Applied Optics
JF - Applied Optics
SN - 0003-6935
IS - 11
ER -