Spectroscopic characterization of the calmodulin-binding and autoinhibitory domains of calcium/calmodulin-dependent protein kinase I

Tao Yuan, Aldrin V Gomes, Junor A. Barnes, Howard N. Hunter, Hans J. Vogel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Structural studies of the calmodulin-dependent protein kinase I have shown how the calmodulin-binding domain and autoinhibitory domain interact with the active sites of the enzyme. In this work, we have studied the interaction in solution of two synthetic short and long (22- and 37-residue) peptides representing the binding and autoinhibitory domains of CaMKI with Ca 2+-CaM using CD, NMR, and EPR spectroscopy. Both peptides adopt α-helical structure when bound to Ca2+-CaM, as detected by CD spectroscopy. Cadmium-113 NMR showed that both peptides induced cooperativity in metal ion binding between the two lobes of the protein. To directly observe the effect of the peptides upon CaM in solution, biosynthetically isotope labeled [methyl-13C-Met]CaM was prepared and studied by 1H, 13C NMR. The relaxation effects of two nitroxide spin-labeled derivatives of the short peptide showed the N-terminal portion of the CaM-binding domain interacting with the C-lobe of CaM, while the C-lobe of the peptide binds to the N-lobe of CaM. Our results are consistent with Trp303 and Met316 acting as the anchoring residues for the C- and N-lobes of CaM, respectively. The NMR spectra of the long peptide showed further differences, suggesting that additional interactions may exist between the autoinhibitory domain and CaM.

Original languageEnglish (US)
Pages (from-to)192-206
Number of pages15
JournalArchives of Biochemistry and Biophysics
Volume421
Issue number2
DOIs
StatePublished - Jan 15 2004
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinase Type 1
Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Peptides
Nuclear magnetic resonance
Spectroscopy
Cadmium
Isotopes
Metal ions
Paramagnetic resonance
Catalytic Domain
Spectrum Analysis
Magnetic Resonance Spectroscopy
Metals
Ions
Derivatives

Keywords

  • Calmodulin
  • Calmodulin-dependent protein kinase I
  • Circular dichroism
  • Electron paramagnetic resonance
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Spectroscopic characterization of the calmodulin-binding and autoinhibitory domains of calcium/calmodulin-dependent protein kinase I. / Yuan, Tao; Gomes, Aldrin V; Barnes, Junor A.; Hunter, Howard N.; Vogel, Hans J.

In: Archives of Biochemistry and Biophysics, Vol. 421, No. 2, 15.01.2004, p. 192-206.

Research output: Contribution to journalArticle

Yuan, Tao ; Gomes, Aldrin V ; Barnes, Junor A. ; Hunter, Howard N. ; Vogel, Hans J. / Spectroscopic characterization of the calmodulin-binding and autoinhibitory domains of calcium/calmodulin-dependent protein kinase I. In: Archives of Biochemistry and Biophysics. 2004 ; Vol. 421, No. 2. pp. 192-206.
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