Spectrophotometric Substrates for Cytosolic Epoxide Hydrolase

E. C. Dietze, E. Kuwano, B. D. Hammock

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In this study, we demonstrate the utility of a broad class of spectrophotometric substrates for the assay of cytosolic epoxide hydrolase purified from murine liver. These substrates, epoxy esters or carbonates, cyclize spontaneously upon or during hydrolysis of the epoxide functionality. The alcohol released by cyclization may then be assayed directly or by coupling to a second reaction. The alcohol produced, or its secondary reaction products, can be selected to give an absorption in the visible or near-uv range of the spectrum. This allows the synthesis of a wide variety of useful spectrophotometric substrates. 4-Nitrophenyl (2S,3S)-2,3-epoxy-3-phenylpropyl carbonate, at pH 6.4 and 25°C, had a Vmax of 22 μmol min-1 mg-1 and a Km of 16 μM when assayed with a conventional spectrophotometer. When assayed under the same conditions with a 96-well plate reader, the measured Vmax was 15 μmol min-1 mg-1 and the Km was 6.6 μM. Some of these compounds were also found to be substrates for glutathione S-transferase, microsomal epoxide hydrolase, and porcine liver carboxylesterase. Indeed, 4-nitrophenyl 3,4-epoxy-3-phenylbutanoate was a 3.4-fold better substrate for porcine liver carboxylesterase than 4-nitrophenyl acetate when initial rates of hydrolysis were measured under the same conditions.

Original languageEnglish (US)
Pages (from-to)176-187
Number of pages12
JournalAnalytical Biochemistry
Volume216
Issue number1
DOIs
StatePublished - Jan 1994

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Epoxide Hydrolases
Carboxylesterase
Carbonates
Liver
Hydrolysis
Substrates
Swine
Alcohols
Epoxy Compounds
Cyclization
Glutathione Transferase
Esters
Spectrophotometers
Reaction products
Assays
4-nitrophenyl

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Spectrophotometric Substrates for Cytosolic Epoxide Hydrolase. / Dietze, E. C.; Kuwano, E.; Hammock, B. D.

In: Analytical Biochemistry, Vol. 216, No. 1, 01.1994, p. 176-187.

Research output: Contribution to journalArticle

Dietze, E. C. ; Kuwano, E. ; Hammock, B. D. / Spectrophotometric Substrates for Cytosolic Epoxide Hydrolase. In: Analytical Biochemistry. 1994 ; Vol. 216, No. 1. pp. 176-187.
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