Spectrophotometric assay for mammalian cytosolic epoxide hydrolase using trans-stilbene oxide as the substrate

Leslie S. Hasegawa, Bruce D. Hammock

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

A continuous spectrophotometric assay based on the differences in the ultraviolet spectra of trans-stilbene oxide (TSO) and its reaction product l,2-diphenyl-l,2-ethanediol is described for the measurement of mammalian cytosolic epoxide hydrolase activity. Rates of TSO hydration determined by this method were similar to those obtained by independent analytical methods, and the assay is rapid, reproducible and relatively free from interference.

Original languageEnglish (US)
Pages (from-to)1979-1984
Number of pages6
JournalBiochemical Pharmacology
Volume31
Issue number11
DOIs
StatePublished - Jun 1 1982

ASJC Scopus subject areas

  • Pharmacology

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