Spectrophotometric assay for mammalian cytosolic epoxide hydrolase using trans-stilbene oxide as the substrate

Leslie S. Hasegawa, Bruce D. Hammock

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A continuous spectrophotometric assay based on the differences in the ultraviolet spectra of trans-stilbene oxide (TSO) and its reaction product l,2-diphenyl-l,2-ethanediol is described for the measurement of mammalian cytosolic epoxide hydrolase activity. Rates of TSO hydration determined by this method were similar to those obtained by independent analytical methods, and the assay is rapid, reproducible and relatively free from interference.

Original languageEnglish (US)
Pages (from-to)1979-1984
Number of pages6
JournalBiochemical Pharmacology
Volume31
Issue number11
DOIs
StatePublished - Jun 1 1982

Fingerprint

Epoxide Hydrolases
Ethylene Glycols
Assays
Substrates
Reaction products
Hydration
stilbene oxide
diphenyl

ASJC Scopus subject areas

  • Pharmacology

Cite this

Spectrophotometric assay for mammalian cytosolic epoxide hydrolase using trans-stilbene oxide as the substrate. / Hasegawa, Leslie S.; Hammock, Bruce D.

In: Biochemical Pharmacology, Vol. 31, No. 11, 01.06.1982, p. 1979-1984.

Research output: Contribution to journalArticle

@article{2039e0f0a615414b93fba2835b1e1cf3,
title = "Spectrophotometric assay for mammalian cytosolic epoxide hydrolase using trans-stilbene oxide as the substrate",
abstract = "A continuous spectrophotometric assay based on the differences in the ultraviolet spectra of trans-stilbene oxide (TSO) and its reaction product l,2-diphenyl-l,2-ethanediol is described for the measurement of mammalian cytosolic epoxide hydrolase activity. Rates of TSO hydration determined by this method were similar to those obtained by independent analytical methods, and the assay is rapid, reproducible and relatively free from interference.",
author = "Hasegawa, {Leslie S.} and Hammock, {Bruce D.}",
year = "1982",
month = "6",
day = "1",
doi = "10.1016/0006-2952(82)90408-7",
language = "English (US)",
volume = "31",
pages = "1979--1984",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier Inc.",
number = "11",

}

TY - JOUR

T1 - Spectrophotometric assay for mammalian cytosolic epoxide hydrolase using trans-stilbene oxide as the substrate

AU - Hasegawa, Leslie S.

AU - Hammock, Bruce D.

PY - 1982/6/1

Y1 - 1982/6/1

N2 - A continuous spectrophotometric assay based on the differences in the ultraviolet spectra of trans-stilbene oxide (TSO) and its reaction product l,2-diphenyl-l,2-ethanediol is described for the measurement of mammalian cytosolic epoxide hydrolase activity. Rates of TSO hydration determined by this method were similar to those obtained by independent analytical methods, and the assay is rapid, reproducible and relatively free from interference.

AB - A continuous spectrophotometric assay based on the differences in the ultraviolet spectra of trans-stilbene oxide (TSO) and its reaction product l,2-diphenyl-l,2-ethanediol is described for the measurement of mammalian cytosolic epoxide hydrolase activity. Rates of TSO hydration determined by this method were similar to those obtained by independent analytical methods, and the assay is rapid, reproducible and relatively free from interference.

UR - http://www.scopus.com/inward/record.url?scp=0019967608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019967608&partnerID=8YFLogxK

U2 - 10.1016/0006-2952(82)90408-7

DO - 10.1016/0006-2952(82)90408-7

M3 - Article

C2 - 7115419

AN - SCOPUS:0019967608

VL - 31

SP - 1979

EP - 1984

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 11

ER -