Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography

Larry Kagemann, Gadi Wollstein, Maciej Wojtkowski, Hiroshi Ishikawa, Kelly A. Townsend, Michelle L. Gabriele, Vivek Srinivasan, James G. Fujimoto, Joel S. Schuman

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

We use Fourier domain optical coherence tomography (OCT) data to assess retinal blood oxygen saturation. Three-dimensional disk-centered retinal tissue volumes were assessed in 17 normal healthy subjects. After removing DC and low-frequency a-scan components, an OCT fundus image was created by integrating total reflectance into a single reflectance value. Thirty fringe patterns were sampled; 10 each from the edge of an artery, adjacent tissue, and the edge of a vein, respectively. A-scans were recalculated, zeroing the DC term in the power spectrum, and used for analysis. Optical density ratios (ODRs) were calculated as ODRArt =ln(Tissue855/ Art855) / ln(Tissue805/ Art805) and ODRVein =ln(Tissue855/Vein855) / ln(Tissue805/Vein805) with Tissue, Art, and Vein representing total a-scan reflectance at the 805- or 855-nm centered bandwidth. Arterial and venous ODRs were compared by the Wilcoxon signed rank test. Arterial ODRs were significantly greater than venous ODRs (1.007±2.611 and -1.434±4.310, respectively; p=0.0217) (mean±standard deviation). A difference between arterial and venous blood saturation was detected. This suggests that retinal oximetry may possibly be added as a metabolic measurement in structural imaging devices.

Original languageEnglish (US)
Article number041212
JournalJournal of Biomedical Optics
Volume12
Issue number4
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

Keywords

  • Metabolism
  • Optical coherence tomography
  • Oximetry
  • Oxygen
  • Retina
  • Spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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    Kagemann, L., Wollstein, G., Wojtkowski, M., Ishikawa, H., Townsend, K. A., Gabriele, M. L., Srinivasan, V., Fujimoto, J. G., & Schuman, J. S. (2007). Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography. Journal of Biomedical Optics, 12(4), [041212]. https://doi.org/10.1117/1.2772655