Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography

Larry Kagemann, Gadi Wollstein, Maciej Wojtkowski, Hiroshi Ishikawa, Kelly A. Townsend, Michelle L. Gabriele, Vivek Srinivasan, James G. Fujimoto, Joel S. Schuman

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We use Fourier domain optical coherence tomography (OCT) data to assess retinal blood oxygen saturation. Three-dimensional disk-centered retinal tissue volumes were assessed in 17 normal healthy subjects. After removing DC and low-frequency a-scan components, an OCT fundus image was created by integrating total reflectance into a single reflectance value. Thirty fringe patterns were sampled; 10 each from the edge of an artery, adjacent tissue, and the edge of a vein, respectively. A-scans were recalculated, zeroing the DC term in the power spectrum, and used for analysis. Optical density ratios (ODRs) were calculated as ODRArt =ln(Tissue855/ Art855) / ln(Tissue805/ Art805) and ODRVein =ln(Tissue855/Vein855) / ln(Tissue805/Vein805) with Tissue, Art, and Vein representing total a-scan reflectance at the 805- or 855-nm centered bandwidth. Arterial and venous ODRs were compared by the Wilcoxon signed rank test. Arterial ODRs were significantly greater than venous ODRs (1.007±2.611 and -1.434±4.310, respectively; p=0.0217) (mean±standard deviation). A difference between arterial and venous blood saturation was detected. This suggests that retinal oximetry may possibly be added as a metabolic measurement in structural imaging devices.

Original languageEnglish (US)
Article number041212
JournalJournal of Biomedical Optics
Volume12
Issue number4
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

Fingerprint

oximetry
Density (optical)
Optical tomography
optical density
tomography
high speed
Tissue
reflectance
veins
blood
Blood
direct current
rank tests
saturation
arts
arteries
Power spectrum
power spectra
diffraction patterns
Oxygen

Keywords

  • Metabolism
  • Optical coherence tomography
  • Oximetry
  • Oxygen
  • Retina
  • Spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Kagemann, L., Wollstein, G., Wojtkowski, M., Ishikawa, H., Townsend, K. A., Gabriele, M. L., ... Schuman, J. S. (2007). Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography. Journal of Biomedical Optics, 12(4), [041212]. https://doi.org/10.1117/1.2772655

Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography. / Kagemann, Larry; Wollstein, Gadi; Wojtkowski, Maciej; Ishikawa, Hiroshi; Townsend, Kelly A.; Gabriele, Michelle L.; Srinivasan, Vivek; Fujimoto, James G.; Schuman, Joel S.

In: Journal of Biomedical Optics, Vol. 12, No. 4, 041212, 01.07.2007.

Research output: Contribution to journalArticle

Kagemann, L, Wollstein, G, Wojtkowski, M, Ishikawa, H, Townsend, KA, Gabriele, ML, Srinivasan, V, Fujimoto, JG & Schuman, JS 2007, 'Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography', Journal of Biomedical Optics, vol. 12, no. 4, 041212. https://doi.org/10.1117/1.2772655
Kagemann L, Wollstein G, Wojtkowski M, Ishikawa H, Townsend KA, Gabriele ML et al. Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography. Journal of Biomedical Optics. 2007 Jul 1;12(4). 041212. https://doi.org/10.1117/1.2772655
Kagemann, Larry ; Wollstein, Gadi ; Wojtkowski, Maciej ; Ishikawa, Hiroshi ; Townsend, Kelly A. ; Gabriele, Michelle L. ; Srinivasan, Vivek ; Fujimoto, James G. ; Schuman, Joel S. / Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography. In: Journal of Biomedical Optics. 2007 ; Vol. 12, No. 4.
@article{bed0dc75cf9341788f4ecdfd2865615c,
title = "Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography",
abstract = "We use Fourier domain optical coherence tomography (OCT) data to assess retinal blood oxygen saturation. Three-dimensional disk-centered retinal tissue volumes were assessed in 17 normal healthy subjects. After removing DC and low-frequency a-scan components, an OCT fundus image was created by integrating total reflectance into a single reflectance value. Thirty fringe patterns were sampled; 10 each from the edge of an artery, adjacent tissue, and the edge of a vein, respectively. A-scans were recalculated, zeroing the DC term in the power spectrum, and used for analysis. Optical density ratios (ODRs) were calculated as ODRArt =ln(Tissue855/ Art855) / ln(Tissue805/ Art805) and ODRVein =ln(Tissue855/Vein855) / ln(Tissue805/Vein805) with Tissue, Art, and Vein representing total a-scan reflectance at the 805- or 855-nm centered bandwidth. Arterial and venous ODRs were compared by the Wilcoxon signed rank test. Arterial ODRs were significantly greater than venous ODRs (1.007±2.611 and -1.434±4.310, respectively; p=0.0217) (mean±standard deviation). A difference between arterial and venous blood saturation was detected. This suggests that retinal oximetry may possibly be added as a metabolic measurement in structural imaging devices.",
keywords = "Metabolism, Optical coherence tomography, Oximetry, Oxygen, Retina, Spectroscopy",
author = "Larry Kagemann and Gadi Wollstein and Maciej Wojtkowski and Hiroshi Ishikawa and Townsend, {Kelly A.} and Gabriele, {Michelle L.} and Vivek Srinivasan and Fujimoto, {James G.} and Schuman, {Joel S.}",
year = "2007",
month = "7",
day = "1",
doi = "10.1117/1.2772655",
language = "English (US)",
volume = "12",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "4",

}

TY - JOUR

T1 - Spectral oximetry assessed with high-speed ultra-highresolution optical coherence tomography

AU - Kagemann, Larry

AU - Wollstein, Gadi

AU - Wojtkowski, Maciej

AU - Ishikawa, Hiroshi

AU - Townsend, Kelly A.

AU - Gabriele, Michelle L.

AU - Srinivasan, Vivek

AU - Fujimoto, James G.

AU - Schuman, Joel S.

PY - 2007/7/1

Y1 - 2007/7/1

N2 - We use Fourier domain optical coherence tomography (OCT) data to assess retinal blood oxygen saturation. Three-dimensional disk-centered retinal tissue volumes were assessed in 17 normal healthy subjects. After removing DC and low-frequency a-scan components, an OCT fundus image was created by integrating total reflectance into a single reflectance value. Thirty fringe patterns were sampled; 10 each from the edge of an artery, adjacent tissue, and the edge of a vein, respectively. A-scans were recalculated, zeroing the DC term in the power spectrum, and used for analysis. Optical density ratios (ODRs) were calculated as ODRArt =ln(Tissue855/ Art855) / ln(Tissue805/ Art805) and ODRVein =ln(Tissue855/Vein855) / ln(Tissue805/Vein805) with Tissue, Art, and Vein representing total a-scan reflectance at the 805- or 855-nm centered bandwidth. Arterial and venous ODRs were compared by the Wilcoxon signed rank test. Arterial ODRs were significantly greater than venous ODRs (1.007±2.611 and -1.434±4.310, respectively; p=0.0217) (mean±standard deviation). A difference between arterial and venous blood saturation was detected. This suggests that retinal oximetry may possibly be added as a metabolic measurement in structural imaging devices.

AB - We use Fourier domain optical coherence tomography (OCT) data to assess retinal blood oxygen saturation. Three-dimensional disk-centered retinal tissue volumes were assessed in 17 normal healthy subjects. After removing DC and low-frequency a-scan components, an OCT fundus image was created by integrating total reflectance into a single reflectance value. Thirty fringe patterns were sampled; 10 each from the edge of an artery, adjacent tissue, and the edge of a vein, respectively. A-scans were recalculated, zeroing the DC term in the power spectrum, and used for analysis. Optical density ratios (ODRs) were calculated as ODRArt =ln(Tissue855/ Art855) / ln(Tissue805/ Art805) and ODRVein =ln(Tissue855/Vein855) / ln(Tissue805/Vein805) with Tissue, Art, and Vein representing total a-scan reflectance at the 805- or 855-nm centered bandwidth. Arterial and venous ODRs were compared by the Wilcoxon signed rank test. Arterial ODRs were significantly greater than venous ODRs (1.007±2.611 and -1.434±4.310, respectively; p=0.0217) (mean±standard deviation). A difference between arterial and venous blood saturation was detected. This suggests that retinal oximetry may possibly be added as a metabolic measurement in structural imaging devices.

KW - Metabolism

KW - Optical coherence tomography

KW - Oximetry

KW - Oxygen

KW - Retina

KW - Spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=35948939354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35948939354&partnerID=8YFLogxK

U2 - 10.1117/1.2772655

DO - 10.1117/1.2772655

M3 - Article

C2 - 17867801

AN - SCOPUS:35948939354

VL - 12

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 4

M1 - 041212

ER -