Spectral imaging for brightfield microscopy

Richard M Levenson; Paul J. Cronin; Kirill K. Pankratov

doi:10.1117/12.485550

Spectral imaging for brightfield microscopy

Richard M Levenson, Paul J. Cronin, Kirill K. Pankratov

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

16 Scopus citations

Abstract

Spectral imaging, whether accomplished using liquid crystal tunable filters, especially agile sources, or other techniques, significantly outperforms color analysis approaches based on RGB imaging systems in samples relevant to current pathology practice. How much spectral resolving power is actually needed remains to be determined, but currently it appears that, up to a point, more spectral images are better, improving the ability of the present algorithms to separate overlapping signals with minimal cross-talk.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	R.M. Levenson, G.H. Bearman, A. Mahadevan-Jansen
Pages	27-33
Number of pages	7
Volume	4959
DOIs	https://doi.org/10.1117/12.485550
State	Published - 2003
Externally published	Yes
Event	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Spectral Imaging: Instrumentation, Applications. and Analysis II - San Jose, CA, United States Duration: Jan 26 2003 → Jan 26 2003

Other

Other	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Spectral Imaging: Instrumentation, Applications. and Analysis II
Country/Territory	United States
City	San Jose, CA
Period	1/26/03 → 1/26/03

Keywords

Chromogens
Illumination
Imaging
Immunohistoc hemistry
Segmentation
Spectral
Tunable
Unmixing

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

Access to Document

10.1117/12.485550

Cite this

Levenson, RM, Cronin, PJ & Pankratov, KK 2003, Spectral imaging for brightfield microscopy. in RM Levenson, GH Bearman & A Mahadevan-Jansen (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4959, pp. 27-33, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Spectral Imaging: Instrumentation, Applications. and Analysis II, San Jose, CA, United States, 1/26/03. https://doi.org/10.1117/12.485550

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abstract = "Spectral imaging, whether accomplished using liquid crystal tunable filters, especially agile sources, or other techniques, significantly outperforms color analysis approaches based on RGB imaging systems in samples relevant to current pathology practice. How much spectral resolving power is actually needed remains to be determined, but currently it appears that, up to a point, more spectral images are better, improving the ability of the present algorithms to separate overlapping signals with minimal cross-talk.",

keywords = "Chromogens, Illumination, Imaging, Immunohistoc hemistry, Segmentation, Spectral, Tunable, Unmixing",

author = "Levenson, {Richard M} and Cronin, {Paul J.} and Pankratov, {Kirill K.}",

year = "2003",

doi = "10.1117/12.485550",

language = "English (US)",

volume = "4959",

pages = "27--33",

editor = "R.M. Levenson and G.H. Bearman and A. Mahadevan-Jansen",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Spectral Imaging: Instrumentation, Applications. and Analysis II ; Conference date: 26-01-2003 Through 26-01-2003",

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AU - Cronin, Paul J.

AU - Pankratov, Kirill K.

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AB - Spectral imaging, whether accomplished using liquid crystal tunable filters, especially agile sources, or other techniques, significantly outperforms color analysis approaches based on RGB imaging systems in samples relevant to current pathology practice. How much spectral resolving power is actually needed remains to be determined, but currently it appears that, up to a point, more spectral images are better, improving the ability of the present algorithms to separate overlapping signals with minimal cross-talk.

KW - Chromogens

KW - Illumination

KW - Imaging

KW - Immunohistoc hemistry

KW - Segmentation

KW - Spectral

KW - Tunable

KW - Unmixing

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DO - 10.1117/12.485550

M3 - Conference contribution

AN - SCOPUS:0344119587

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EP - 33

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Levenson, R.M.

A2 - Bearman, G.H.

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Y2 - 26 January 2003 through 26 January 2003

ER -

Spectral imaging for brightfield microscopy

Abstract

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Keywords

ASJC Scopus subject areas

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