Spectral imaging for brightfield microscopy

Richard M Levenson, Paul J. Cronin, Kirill K. Pankratov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

16 Scopus citations

Abstract

Spectral imaging, whether accomplished using liquid crystal tunable filters, especially agile sources, or other techniques, significantly outperforms color analysis approaches based on RGB imaging systems in samples relevant to current pathology practice. How much spectral resolving power is actually needed remains to be determined, but currently it appears that, up to a point, more spectral images are better, improving the ability of the present algorithms to separate overlapping signals with minimal cross-talk.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.M. Levenson, G.H. Bearman, A. Mahadevan-Jansen
Pages27-33
Number of pages7
Volume4959
DOIs
StatePublished - 2003
Externally publishedYes
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Spectral Imaging: Instrumentation, Applications. and Analysis II - San Jose, CA, United States
Duration: Jan 26 2003Jan 26 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Spectral Imaging: Instrumentation, Applications. and Analysis II
Country/TerritoryUnited States
CitySan Jose, CA
Period1/26/031/26/03

Keywords

  • Chromogens
  • Illumination
  • Imaging
  • Immunohistoc hemistry
  • Segmentation
  • Spectral
  • Tunable
  • Unmixing

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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