Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits

Barry J. Pate, Kenneth L. White, Quinton A. Winger, Lee F. Rickords, Kenneth I. Aston, Benjamin R. Sessons, Guang Peng Li, Kenneth D. Campbell, Bart C Weimer, Thomas D. Bunch

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Integrins facilitate attachment of cells to the extra-cellular matrix, often binding the arginine-glycine-aspartic acid tri-peptide motif, thus facilitating cell migration, mediating cell-cell adhesion, linking the extracellular matrix (ECM) with cytoskeletal elements, and acting as signaling molecules. Adhesion activates signaling mechanisms that regulate integrin function, cytoskeletal assembly, cell behavior, and protein synthesis. Immunofluorescence was used to determine the presence of integrin α and β subunits on the surface of bovine oocytes using a panel of monoclonal antibodies (mAbs) specific for αL, αM, αX, αV, α2, α4, α6, β1, β2, and β3 antigens, with multiple antibodies for each subunit. Confocal microscopy indicated the presence of αV, α6, α4, α2, β1, and β3 integrin subunits on the plasma membrane of bovine oocytes. The presence of these subunits was verified by RT-PCR analysis using primers designed based on known gene sequences of bovine integrin subunits, or by using sequence information using bovine expressed sequence tags (EST) compared with known human and murine integrin subunit gene sequence information. Previously unpublished sequence information for bovine α6 and β3 integrins was determined. The presence of these integrin subunits on the bovine oocyte vitelline membrane supports the hypothesis that sperm-oocyte interactions in the bovine are mediated by integrins.

Original languageEnglish (US)
Pages (from-to)600-607
Number of pages8
JournalMolecular Reproduction and Development
Volume74
Issue number5
DOIs
StatePublished - May 2007
Externally publishedYes

Fingerprint

Integrins
Oocytes
Vitelline Membrane
Sperm-Ovum Interactions
Expressed Sequence Tags
Aspartic Acid
Cell Adhesion
Confocal Microscopy
Glycine
Genes
Cell Movement
Fluorescent Antibody Technique
Extracellular Matrix
Arginine
Monoclonal Antibodies
Cell Membrane
Antigens
Polymerase Chain Reaction
Peptides
Antibodies

Keywords

  • Activation
  • Bovine
  • Integrin
  • Oocyte
  • Receptor

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Pate, B. J., White, K. L., Winger, Q. A., Rickords, L. F., Aston, K. I., Sessons, B. R., ... Bunch, T. D. (2007). Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits. Molecular Reproduction and Development, 74(5), 600-607. https://doi.org/10.1002/mrd.20649

Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits. / Pate, Barry J.; White, Kenneth L.; Winger, Quinton A.; Rickords, Lee F.; Aston, Kenneth I.; Sessons, Benjamin R.; Li, Guang Peng; Campbell, Kenneth D.; Weimer, Bart C; Bunch, Thomas D.

In: Molecular Reproduction and Development, Vol. 74, No. 5, 05.2007, p. 600-607.

Research output: Contribution to journalArticle

Pate, BJ, White, KL, Winger, QA, Rickords, LF, Aston, KI, Sessons, BR, Li, GP, Campbell, KD, Weimer, BC & Bunch, TD 2007, 'Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits', Molecular Reproduction and Development, vol. 74, no. 5, pp. 600-607. https://doi.org/10.1002/mrd.20649
Pate, Barry J. ; White, Kenneth L. ; Winger, Quinton A. ; Rickords, Lee F. ; Aston, Kenneth I. ; Sessons, Benjamin R. ; Li, Guang Peng ; Campbell, Kenneth D. ; Weimer, Bart C ; Bunch, Thomas D. / Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits. In: Molecular Reproduction and Development. 2007 ; Vol. 74, No. 5. pp. 600-607.
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