Species-specific antagonism of Ah receptor action by 2,2′,5,5′-tetrachloro- and 2,2′,3,3′,4,4′-hexachlorobiphenyl

Jac M M J G Aarts, Michael S. Denison, Mary A. Cox, Marjolijn A C Schalk, Patricia M. Garrison, Kathryn Tullis, Laura H J de Haan, Abraham Brouwer

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Using recombinant cell lines showing Ah receptor-controlled expression of a luciferase reporter gene, the interaction of di-ortho-substituted polychlorinated biphenyls (PCBs) with Ah receptor agonists was studied. In the recombinant Hepa1c1c7 mouse hepatoma (H1L1.1c7) cells strong antagonistic interaction of 2,2′,5,5′-tetrachlorobiphenyl (PCB52) with luciferase expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or 3,3′,4,4′-tetrachlorobiphenyl (PCB77) was observed, and similarly, between 2,2′,3,3′,4,4′-hexachlorobiphenyl (PCB128) and PCB77. Accordingly, PCB52 was found to inhibit ethoxyresorufin-O-deethylase (EROD) induction by PCB77 in wild-type Hepa1c1c7 cells. In contrast, the antagonistic effect of PCB52 on TCDD-induced luciferase expression was only minor in recombinant guinea pig GPC16 colon adenocarcinoma (G16L1.1c8) and human HepG2 hepatoma (HG2L1.1c3) cells, and intemediate in recombinant H4IIE rat hepatoma (H4L1.1c4) cells. Gel retardation studies using a 32P-labelled dioxin responsive element (DRE)-containing oligonucleotide, and ligand binding studies using [3H]TCDD, demonstrated that the species-specific antagonistic activity of PCB52 on Ah receptor-controlled luciferase expression is due to inhibition of Ah receptor ligand and DNA binding. We conclude, that Ah-mediated luciferase expression provides a useful tool to study the species specificity of Ah receptor (ant)agonists.

Original languageEnglish (US)
Pages (from-to)463-474
Number of pages12
JournalEuropean Journal of Pharmacology: Environmental Toxicology and
Volume293
Issue number4
DOIs
StatePublished - Dec 7 1995

Fingerprint

Luciferases
Ligands
Oligonucleotides
Hepatocellular Carcinoma
Polychlorinated biphenyls
Rats
DNA
Gels
Genes
Cells
Species Specificity
Cytochrome P-450 CYP1A1
Dioxins
Ants
Polychlorinated Biphenyls
Reporter Genes
Guinea Pigs
Colon
Adenocarcinoma
2,3,4,2',3',4'-hexachlorobiphenyl

Keywords

  • (Interaction)
  • 2,3,7,8-TCDD
  • Aryl hydrocarbon receptor
  • Biomarker
  • PCB
  • Reporter gene

ASJC Scopus subject areas

  • Pollution
  • Pharmacology
  • Toxicology

Cite this

Species-specific antagonism of Ah receptor action by 2,2′,5,5′-tetrachloro- and 2,2′,3,3′,4,4′-hexachlorobiphenyl. / Aarts, Jac M M J G; Denison, Michael S.; Cox, Mary A.; Schalk, Marjolijn A C; Garrison, Patricia M.; Tullis, Kathryn; de Haan, Laura H J; Brouwer, Abraham.

In: European Journal of Pharmacology: Environmental Toxicology and, Vol. 293, No. 4, 07.12.1995, p. 463-474.

Research output: Contribution to journalArticle

Aarts, Jac M M J G ; Denison, Michael S. ; Cox, Mary A. ; Schalk, Marjolijn A C ; Garrison, Patricia M. ; Tullis, Kathryn ; de Haan, Laura H J ; Brouwer, Abraham. / Species-specific antagonism of Ah receptor action by 2,2′,5,5′-tetrachloro- and 2,2′,3,3′,4,4′-hexachlorobiphenyl. In: European Journal of Pharmacology: Environmental Toxicology and. 1995 ; Vol. 293, No. 4. pp. 463-474.
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abstract = "Using recombinant cell lines showing Ah receptor-controlled expression of a luciferase reporter gene, the interaction of di-ortho-substituted polychlorinated biphenyls (PCBs) with Ah receptor agonists was studied. In the recombinant Hepa1c1c7 mouse hepatoma (H1L1.1c7) cells strong antagonistic interaction of 2,2′,5,5′-tetrachlorobiphenyl (PCB52) with luciferase expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or 3,3′,4,4′-tetrachlorobiphenyl (PCB77) was observed, and similarly, between 2,2′,3,3′,4,4′-hexachlorobiphenyl (PCB128) and PCB77. Accordingly, PCB52 was found to inhibit ethoxyresorufin-O-deethylase (EROD) induction by PCB77 in wild-type Hepa1c1c7 cells. In contrast, the antagonistic effect of PCB52 on TCDD-induced luciferase expression was only minor in recombinant guinea pig GPC16 colon adenocarcinoma (G16L1.1c8) and human HepG2 hepatoma (HG2L1.1c3) cells, and intemediate in recombinant H4IIE rat hepatoma (H4L1.1c4) cells. Gel retardation studies using a 32P-labelled dioxin responsive element (DRE)-containing oligonucleotide, and ligand binding studies using [3H]TCDD, demonstrated that the species-specific antagonistic activity of PCB52 on Ah receptor-controlled luciferase expression is due to inhibition of Ah receptor ligand and DNA binding. We conclude, that Ah-mediated luciferase expression provides a useful tool to study the species specificity of Ah receptor (ant)agonists.",
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AU - Aarts, Jac M M J G

AU - Denison, Michael S.

AU - Cox, Mary A.

AU - Schalk, Marjolijn A C

AU - Garrison, Patricia M.

AU - Tullis, Kathryn

AU - de Haan, Laura H J

AU - Brouwer, Abraham

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AB - Using recombinant cell lines showing Ah receptor-controlled expression of a luciferase reporter gene, the interaction of di-ortho-substituted polychlorinated biphenyls (PCBs) with Ah receptor agonists was studied. In the recombinant Hepa1c1c7 mouse hepatoma (H1L1.1c7) cells strong antagonistic interaction of 2,2′,5,5′-tetrachlorobiphenyl (PCB52) with luciferase expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or 3,3′,4,4′-tetrachlorobiphenyl (PCB77) was observed, and similarly, between 2,2′,3,3′,4,4′-hexachlorobiphenyl (PCB128) and PCB77. Accordingly, PCB52 was found to inhibit ethoxyresorufin-O-deethylase (EROD) induction by PCB77 in wild-type Hepa1c1c7 cells. In contrast, the antagonistic effect of PCB52 on TCDD-induced luciferase expression was only minor in recombinant guinea pig GPC16 colon adenocarcinoma (G16L1.1c8) and human HepG2 hepatoma (HG2L1.1c3) cells, and intemediate in recombinant H4IIE rat hepatoma (H4L1.1c4) cells. Gel retardation studies using a 32P-labelled dioxin responsive element (DRE)-containing oligonucleotide, and ligand binding studies using [3H]TCDD, demonstrated that the species-specific antagonistic activity of PCB52 on Ah receptor-controlled luciferase expression is due to inhibition of Ah receptor ligand and DNA binding. We conclude, that Ah-mediated luciferase expression provides a useful tool to study the species specificity of Ah receptor (ant)agonists.

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