Spatial expression of the alternatively spliced EIIIB and EIIIA segments of fibronectin in the early chicken embryo

John H. Peters, Jack Sechrist, Simone Luetolf, Grace Loredo, Marianne Bronner-Fraser

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using domain-specific antibodies, we have analyzed the tissue distribution of fibronectins (FNs) containing the alternatively spliced EIIIB and EIIIA segments relative to total FN in early chicken embryos. The results show a selective loss of EIIIA+ FN staining in the notochordal sheath and in cartilaginous structures between 4.5 and 7.0 days of development. In other regions, EIIIB+ and EIIIA+ FNs are extensively codistributed in and around mesoderm-derived structures (somites, notochord, heart, and blood vessels), in basal laminae of endoderm and ectoderm-derived structures, as well as within the vicinity of neural crest formation and migration. We also noted that EIIIA staining overlaps with spatial patterns of distribution that have previously been described for the α4 integrin subunit, a component of the EIIIA receptor α4β1.

Original languageEnglish (US)
Pages (from-to)221-238
Number of pages18
JournalCell Communication and Adhesion
Volume9
Issue number4
StatePublished - Jul 2002
Externally publishedYes

Fingerprint

Fibronectins
Chickens
Embryonic Structures
Staining and Labeling
Notochord
Somites
Endoderm
Ectoderm
Neural Crest
Blood vessels
Mesoderm
Tissue Distribution
Basement Membrane
Integrins
Blood Vessels
Tissue
Antibodies

Keywords

  • Alternative splicing
  • Chicken embryo
  • Development
  • Extracellular matrix
  • Fibronectin
  • Neural crest

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry

Cite this

Peters, J. H., Sechrist, J., Luetolf, S., Loredo, G., & Bronner-Fraser, M. (2002). Spatial expression of the alternatively spliced EIIIB and EIIIA segments of fibronectin in the early chicken embryo. Cell Communication and Adhesion, 9(4), 221-238.

Spatial expression of the alternatively spliced EIIIB and EIIIA segments of fibronectin in the early chicken embryo. / Peters, John H.; Sechrist, Jack; Luetolf, Simone; Loredo, Grace; Bronner-Fraser, Marianne.

In: Cell Communication and Adhesion, Vol. 9, No. 4, 07.2002, p. 221-238.

Research output: Contribution to journalArticle

Peters, JH, Sechrist, J, Luetolf, S, Loredo, G & Bronner-Fraser, M 2002, 'Spatial expression of the alternatively spliced EIIIB and EIIIA segments of fibronectin in the early chicken embryo', Cell Communication and Adhesion, vol. 9, no. 4, pp. 221-238.
Peters, John H. ; Sechrist, Jack ; Luetolf, Simone ; Loredo, Grace ; Bronner-Fraser, Marianne. / Spatial expression of the alternatively spliced EIIIB and EIIIA segments of fibronectin in the early chicken embryo. In: Cell Communication and Adhesion. 2002 ; Vol. 9, No. 4. pp. 221-238.
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