SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells

Smita Tanpure, Jerusha Boyineini, Manu Gnanamony, Reuben Antony, Karen S. Fernández, Jaime Libes, Julian Lin, David Pinson, Pushpa A. Joseph, Christopher S. Gondi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27) in vivo and suppresses mitochondrial membrane potential (Δψ) in neuroblastoma cells. In the present study, the overexpression of SPARC in SK-N-BE(2) and NB1691 neuroblastoma cell lines suppresses radiation-induced G2M cell cycle arrest, proliferation, HSP27 expression (in vitro and in vivo) and induces the collapse of the mitochondrial Δψ. Gene ontology analysis demonstrated that the overexpression of SPARC combined with irradiation, induces the expression of dissimilar molecular function genes in SK-N-BE(2) and NB1691 cells, providing evidence of a dissimilar response signaling pathway. These results demonstrate that overexpression of SPARC suppresses radiation-induced HSP27 expression in neuroblastoma cells and the combination of SPARC and radiation induces the expression of protein 21, but suppresses neuroblastoma tumor density in in vivo mouse models. SPARC also induces mitochondrial Δψ collapse in SK-N-BE(2) and NB1691 neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)4602-4610
Number of pages9
JournalOncology Letters
Volume13
Issue number6
DOIs
StatePublished - Jun 1 2017
Externally publishedYes

Fingerprint

HSP27 Heat-Shock Proteins
Neuroblastoma
Cysteine
Radiation
Proteins
Child Mortality
Gene Ontology
Mitochondrial Membrane Potential
Cell Cycle Checkpoints
Neoplasms
Survival Rate
Cell Line

Keywords

  • HSP27
  • Mitochondria
  • Neuroblastoma
  • Radiation
  • SPARC

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells. / Tanpure, Smita; Boyineini, Jerusha; Gnanamony, Manu; Antony, Reuben; Fernández, Karen S.; Libes, Jaime; Lin, Julian; Pinson, David; Joseph, Pushpa A.; Gondi, Christopher S.

In: Oncology Letters, Vol. 13, No. 6, 01.06.2017, p. 4602-4610.

Research output: Contribution to journalArticle

Tanpure, S, Boyineini, J, Gnanamony, M, Antony, R, Fernández, KS, Libes, J, Lin, J, Pinson, D, Joseph, PA & Gondi, CS 2017, 'SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells', Oncology Letters, vol. 13, no. 6, pp. 4602-4610. https://doi.org/10.3892/ol.2017.6075
Tanpure, Smita ; Boyineini, Jerusha ; Gnanamony, Manu ; Antony, Reuben ; Fernández, Karen S. ; Libes, Jaime ; Lin, Julian ; Pinson, David ; Joseph, Pushpa A. ; Gondi, Christopher S. / SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial Δψ in neuroblastoma cells. In: Oncology Letters. 2017 ; Vol. 13, No. 6. pp. 4602-4610.
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