Sox9 neural crest determinant gene controls patterning and closure of the posterior frontal cranial suture

David E Sahar, Michael T. Longaker, Natalina Quarto

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Cranial suture development involves a complex interaction of genes and tissues derived from neural crest cells (NCC) and paraxial mesoderm. In mice, the posterior frontal (PF) suture closes during the first month of life while other sutures remain patent throughout the life of the animal. Given the unique NCC origin of PF suture complex (analogous to metopic suture in humans), we performed quantitative real-time PCR and immunohistochemistry to study the expression pattern of the NCC determinant gene Sox9 and select markers of extracellular matrix. Our results indicated a unique up-regulated expression of Sox9, a regulator of chondrogenesis, during initiation of PF suture closure, along with the expression of specific cartilage markers (Type II Collagen and Type X Collagen), as well as cartilage tissue formation in the PF suture. This process was followed by expression of bone markers (Type I Collagen and Osteocalcin), suggesting endochondral ossification. Moreover, we studied the effect of haploinsufficiency of the NCC determinant gene Sox9 in the NCC derived PF suture complex. A decrease in dosage of Sox9 by haploinsufficiency in NCC-derived tissues resulted in delayed PF suture closure. These results demonstrate a unique development of the PF suture complex and the role of Sox9 as an important contributor to timely and proper closure of the PF suture through endochondral ossification.

Original languageEnglish (US)
Pages (from-to)344-361
Number of pages18
JournalDevelopmental Biology
Volume280
Issue number2
DOIs
StatePublished - Apr 15 2005
Externally publishedYes

Fingerprint

Cranial Sutures
Neural Crest
Sutures
Genes
Haploinsufficiency
Osteogenesis
Cartilage
Collagen Type X
Chondrogenesis
Osteocalcin
Mesoderm
Collagen Type I
Extracellular Matrix
Real-Time Polymerase Chain Reaction
Collagen
Immunohistochemistry

Keywords

  • Chondrogenesis
  • Cranial suture patterning
  • Endochondral ossification
  • Neural crest cells
  • Posterior frontal suture
  • Sox9

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Sox9 neural crest determinant gene controls patterning and closure of the posterior frontal cranial suture. / Sahar, David E; Longaker, Michael T.; Quarto, Natalina.

In: Developmental Biology, Vol. 280, No. 2, 15.04.2005, p. 344-361.

Research output: Contribution to journalArticle

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