Sox6 is required for normal fiber type differentiation of fetal skeletal muscle in mice

Nobuko Hagiwara, Michael Yeh, Ann Liu

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

Sox6, a member of the Sox family of transcription factors, is highly expressed in skeletal muscle. Despite its abundant expression, the role of Sox6 in muscle development is not well understood. We hypothesize that, in fetal muscle, Sox6 functions as a repressor of slow fiber type-specific genes. In the wild-type mouse, differentiation of fast and slow fibers becomes apparent during late fetal stages (after approximately embryonic day 16). However, in the Sox6 null-p100H mutant mouse, all fetal muscle fibers maintain slow fiber characteristics, as evidenced by expression of the slow myosin heavy chain MyHC-β. Knockdown of Sox6 expression in wild-type myotubes results in a significant increase in MyHC-β expression, supporting our hypothesis. Analysis of the MyHC-β promoter revealed a Sox consensus sequence that likely functions as a negative cis-regulatory element. Together, our results suggest that Sox6 plays a critical role in the fiber type differentiation of fetal skeletal muscle.

Original languageEnglish (US)
Pages (from-to)2062-2076
Number of pages15
JournalDevelopmental Dynamics
Volume236
Issue number8
DOIs
StatePublished - Aug 2007

Keywords

  • Fetal skeletal muscle
  • Muscle fiber types
  • Sox6
  • Terminal differentiation
  • Transcription

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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