Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization

Dong Wang, Aijun Wang, Fan Wu, Xuefeng Qiu, Ye Li, Julia Chu, Wen Chin Huang, Kang Xu, Xiaohua Gong, Song Li

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Implanted biomaterials and biomedical devices generally induce foreign body reaction and end up with encapsulation by a dense avascular fibrous layer enriched in extracellular matrix. Fibroblasts/myofibroblasts are thought to be the major cell type involved in encapsulation, but it is unclear whether and how stem cells contribute to this process. Here we show, for the first time, that Sox10+ adult stem cells contribute to both encapsulation and microvessel formation. Sox10+ adult stem cells were found sparsely in the stroma of subcutaneous loose connective tissues. Upon subcutaneous biomaterial implantation, Sox10+ stem cells were activated and recruited to the biomaterial scaffold, and differentiated into fibroblasts and then myofibroblasts. This differentiation process from Sox10+ stem cells to myofibroblasts could be recapitulated in vitro. On the other hand, Sox10+ stem cells could differentiate into perivascular cells to stabilize newly formed microvessels. Sox10+ stem cells and endothelial cells in three-dimensional co-culture self-assembled into microvessels, and platelet-derived growth factor had chemotactic effect on Sox10+ stem cells. Transplanted Sox10+ stem cells differentiated into smooth muscle cells to stabilize functional microvessels. These findings demonstrate the critical role of adult stem cells in tissue remodeling and unravel the complexity of stem cell fate determination.

Original languageEnglish (US)
Article number40295
JournalScientific Reports
Volume7
DOIs
StatePublished - Jan 10 2017

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Adult Stem Cells
Biocompatible Materials
Stem Cells
Microvessels
Myofibroblasts
Fibroblasts
Foreign-Body Reaction
Platelet-Derived Growth Factor
Coculture Techniques
Connective Tissue
Smooth Muscle Myocytes
Extracellular Matrix
Endothelial Cells
Equipment and Supplies

ASJC Scopus subject areas

  • General

Cite this

Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization. / Wang, Dong; Wang, Aijun; Wu, Fan; Qiu, Xuefeng; Li, Ye; Chu, Julia; Huang, Wen Chin; Xu, Kang; Gong, Xiaohua; Li, Song.

In: Scientific Reports, Vol. 7, 40295, 10.01.2017.

Research output: Contribution to journalArticle

Wang, D, Wang, A, Wu, F, Qiu, X, Li, Y, Chu, J, Huang, WC, Xu, K, Gong, X & Li, S 2017, 'Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization', Scientific Reports, vol. 7, 40295. https://doi.org/10.1038/srep40295
Wang, Dong ; Wang, Aijun ; Wu, Fan ; Qiu, Xuefeng ; Li, Ye ; Chu, Julia ; Huang, Wen Chin ; Xu, Kang ; Gong, Xiaohua ; Li, Song. / Sox10+ adult stem cells contribute to biomaterial encapsulation and microvascularization. In: Scientific Reports. 2017 ; Vol. 7.
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