Sources and genetic structure of a cluster of genes for resistance to three pathogens in lettuce

H. Witsenboer, R. V. Kesseli, M. G. Fortin, M. Stanghellini, Richard W Michelmore

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

The second largest cluster of resistance genes in lettuce contains at least two downy mildew resistance specificities, Dm5/8 and Dm10, as well as Tu, providing resistance against turnip mosaic virus, and plr, a recessive gene conferring resistance against Plasmopara lactucae-radicis, a root infecting downy mildew. In the present paper four additional genetic markers have been added to this cluster, three RAPD markers and one RFLP marker, CL1795. CL1795 is a member of a multigene family related to triose phosphate isomerase; other members of this family map to the other two major clusters of resistance genes in lettuce. Seven RAPD markers in the region were converted into sequence characterized amplified regions (SCARs) and used in the further analysis of the region and the mapping of Dm10. Three different segregating populations were used to map the four resistance genes relative to molecular markers. There were no significant differences in gene order or rate of recombination between the three crosses. This cluster of resistance genes spans 6.4 cM, with Dm10 1.2 cM from Dm8. Marker analysis of 20 cultivars confirmed multiple origins for Dm5/8 specificity. Two different Lactuca serriola origins for the Du5/8 specificity had previously been described and originally designated as either Dm5 or Dm8. Some ancient cultivars also had the same specificity. Previously, due to lack of recombination in genetic analyses and the same resistance specificities, it was assumed that Dm5 and Dm8 were determined by the same gene. However, molecular marker analysis clearly identified genotypes characteristic of each source. Therefore, Dm5/8 specificity is either ancient and widespread in L. serriola and some L. sativa, or else has arisen on multiple occasions as alleles at the same locus or at linked loci.

Original languageEnglish (US)
Pages (from-to)178-188
Number of pages11
JournalTheoretical And Applied Genetics
Volume91
Issue number1
DOIs
StatePublished - Jul 1995

Keywords

  • Disease resistance
  • Downy mildew
  • Genetic mapping
  • Lettuce
  • Molecular markers

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

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    Witsenboer, H., Kesseli, R. V., Fortin, M. G., Stanghellini, M., & Michelmore, R. W. (1995). Sources and genetic structure of a cluster of genes for resistance to three pathogens in lettuce. Theoretical And Applied Genetics, 91(1), 178-188. https://doi.org/10.1007/BF00220875