Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR

I. Hua Chen; Lien Siang Chou; Shih Jen Chou; Jiann Hsiung Wang; Jeffrey L Stott; Myra Blanchard; I. Fan Jen; Wei Cheng Yang

doi:10.1292/jvms.17-0548

Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR

I. Hua Chen, Lien Siang Chou, Shih Jen Chou, Jiann Hsiung Wang, Jeffrey L Stott, Myra Blanchard, I. Fan Jen, Wei Cheng Yang

Research output: Contribution to journal › Comment/debate › peer-review

3 Scopus citations

Abstract

Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced T_H 2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.

Original language	English (US)
Pages (from-to)	601-605
Number of pages	5
Journal	Journal of Veterinary Medical Science
Volume	80
Issue number	4
DOIs	https://doi.org/10.1292/jvms.17-0548
State	Published - Apr 1 2018

Keywords

Cytokine
Dolphin
Immune response
QRT-PCR
Sound

ASJC Scopus subject areas

veterinary(all)

Access to Document

10.1292/jvms.17-0548

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Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR. / Chen, I. Hua; Chou, Lien Siang; Chou, Shih Jen; Wang, Jiann Hsiung; Stott, Jeffrey L ; Blanchard, Myra; Jen, I. Fan; Yang, Wei Cheng.

In: Journal of Veterinary Medical Science, Vol. 80, No. 4, 01.04.2018, p. 601-605.

Research output: Contribution to journal › Comment/debate › peer-review

Chen, I. Hua ; Chou, Lien Siang ; Chou, Shih Jen ; Wang, Jiann Hsiung ; Stott, Jeffrey L ; Blanchard, Myra ; Jen, I. Fan ; Yang, Wei Cheng. / Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR. In: Journal of Veterinary Medical Science. 2018 ; Vol. 80, No. 4. pp. 601-605.

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abstract = "Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced TH 2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.",

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