Abstract
Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced TH 2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 601-605 |
| Number of pages | 5 |
| Journal | Journal of Veterinary Medical Science |
| Volume | 80 |
| Issue number | 4 |
| DOIs |
|
| State | Published - Apr 1 2018 |
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Keywords
- Cytokine
- Dolphin
- Immune response
- QRT-PCR
- Sound
ASJC Scopus subject areas
- veterinary(all)
Cite this
Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR. / Chen, I. Hua; Chou, Lien Siang; Chou, Shih Jen; Wang, Jiann Hsiung; Stott, Jeffrey L; Blanchard, Myra; Jen, I. Fan; Yang, Wei Cheng.
In: Journal of Veterinary Medical Science, Vol. 80, No. 4, 01.04.2018, p. 601-605.Research output: Contribution to journal › Comment/debate
}
TY - JOUR
T1 - Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR
AU - Chen, I. Hua
AU - Chou, Lien Siang
AU - Chou, Shih Jen
AU - Wang, Jiann Hsiung
AU - Stott, Jeffrey L
AU - Blanchard, Myra
AU - Jen, I. Fan
AU - Yang, Wei Cheng
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced TH 2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.
AB - Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced TH 2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.
KW - Cytokine
KW - Dolphin
KW - Immune response
KW - QRT-PCR
KW - Sound
UR - http://www.scopus.com/inward/record.url?scp=85045691476&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045691476&partnerID=8YFLogxK
U2 - 10.1292/jvms.17-0548
DO - 10.1292/jvms.17-0548
M3 - Comment/debate
C2 - 29479043
AN - SCOPUS:85045691476
VL - 80
SP - 601
EP - 605
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
SN - 0916-7250
IS - 4
ER -