Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR

I. Hua Chen, Lien Siang Chou, Shih Jen Chou, Jiann Hsiung Wang, Jeffrey L Stott, Myra Blanchard, I. Fan Jen, Wei Cheng Yang

Research output: Contribution to journalComment/debate

2 Citations (Scopus)

Abstract

Cetacean health may be potentially affected by anthropogenic sound. We have initiated investigations on the effect of low-frequency underwater sound on immunological gene transcript profiles of captive bottlenose dolphins (Tursiops truncatus) using a probe-based quantitative gene expression assay. Six immunologic genes (IL-2Rα, -4, -10, -12, TNFα and IFNγ) were selected for analysis using two validated housekeeping genes (PGK1 and HPRT1) as reference genes. Twenty-four blood samples from six clinically healthy individuals and six blood samples from individuals after sound exposures were available. The gene transcript profile of sound-exposed dolphins was consistent with a stress-induced TH 2 shift profile as compared to controls. This study may lead to better understanding of the effects of anthropogenic sound on immune responses of cetaceans.

Original languageEnglish (US)
Pages (from-to)601-605
Number of pages5
JournalJournal of Veterinary Medical Science
Volume80
Issue number4
DOIs
StatePublished - Apr 1 2018

Fingerprint

Bottle-Nosed Dolphin
Tursiops truncatus
cytokines
Cytokines
Polymerase Chain Reaction
blood
Genes
genes
Dolphins
Essential Genes
dolphins
Interleukin-4
anthropogenic activities
immune response
Gene Expression
sampling
gene expression
Health
assays

Keywords

  • Cytokine
  • Dolphin
  • Immune response
  • QRT-PCR
  • Sound

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR. / Chen, I. Hua; Chou, Lien Siang; Chou, Shih Jen; Wang, Jiann Hsiung; Stott, Jeffrey L; Blanchard, Myra; Jen, I. Fan; Yang, Wei Cheng.

In: Journal of Veterinary Medical Science, Vol. 80, No. 4, 01.04.2018, p. 601-605.

Research output: Contribution to journalComment/debate

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AU - Wang, Jiann Hsiung

AU - Stott, Jeffrey L

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