Sost, independent of the non-coding enhancer ECR5, is required for bone mechanoadaptation

Alexander G. Robling, Kyung Shin Kang, Whitney A. Bullock, William H. Foster, Deepa Murugesh, Gabriela G. Loots, Damian C Genetos

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Sclerostin (Sost) is a negative regulator of bone formation that acts upon the Wnt signaling pathway. Sost is mechanically regulated at both mRNA and protein level such that loading represses and unloading enhances Sost expression, in osteocytes and in circulation. The non-coding evolutionarily conserved enhancer ECR5 has been previously reported as a transcriptional regulatory element required for modulating Sost expression in osteocytes. Here we explored the mechanisms by which ECR5, or several other putative transcriptional enhancers regulate Sost expression, in response to mechanical stimulation. We found that in vivo ulna loading is equally osteoanabolic in wildtype and Sost−/− mice, although Sost is required for proper distribution of load-induced bone formation to regions of high strain. Using Luciferase reporters carrying the ECR5 non-coding enhancer and heterologous or homologous hSOST promoters, we found that ECR5 is mechanosensitive in vitro and that ECR5-driven Luciferase activity decreases in osteoblasts exposed to oscillatory fluid flow. Yet, ECR5−/− mice showed similar magnitude of load-induced bone formation and similar periosteal distribution of bone formation to high-strain regions compared to wildtype mice. Further, we found that in contrast to Sost−/− mice, which are resistant to disuse-induced bone loss, ECR5−/− mice lose bone upon unloading to a degree similar to wildtype control mice. ECR5 deletion did not abrogate positive effects of unloading on Sost, suggesting that additional transcriptional regulators and regulatory elements contribute to load-induced regulation of Sost.

Original languageEnglish (US)
Pages (from-to)180-188
Number of pages9
JournalBone
Volume92
DOIs
StatePublished - Nov 1 2016

Keywords

  • Disuse
  • ECR5
  • Enhancer
  • Mechanotransduction
  • Sclerostin
  • Skeleton
  • Sost

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Medicine(all)
  • Histology

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  • Cite this

    Robling, A. G., Kang, K. S., Bullock, W. A., Foster, W. H., Murugesh, D., Loots, G. G., & Genetos, D. C. (2016). Sost, independent of the non-coding enhancer ECR5, is required for bone mechanoadaptation. Bone, 92, 180-188. https://doi.org/10.1016/j.bone.2016.09.001